User:Tara K. Luckau/Notebook/Team ConGen/Entry Base

Tara's Lab Notebook

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CNHY Acos5

Pour	Load	Run
270 mL 1x TAE + 5.4 g agarose	2 µL gel load dye + 4 µL PCR product	160 V
27 µL GelRed	6 µL ladder	45 minutes

mispriming everywhere - primer pair not usable for CNHY
lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
favorable conditions indicated by