27 September 2010 - Ordered Primers
- Ordered first set of primers
- Scun2 and Scun22 (highly variable simple motifs)
- Lance et al (2009) Conservation Genetics Resources, Sceloporus undulatus
- Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT)
 - Try all four combinations
- F - R
- F - R pigtail
- F M13 - R
- F M13 - R pigtail
- If they don't hinder efforts of change optimization decisions, then may make optimization of multiplexes cheaper!
11 October 2010 - PCR Thermalcycler Testing
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Tara's Lab Notebook
