User:Tim Hsiau - Revision history

Tim Hsiau: /* Contact Info */

2010-11-20T07:04:04Z

Contact Info

←Older revision		Revision as of 07:04, 20 November 2010
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	[[IGEM:Berkeley/2010]]		[[IGEM:Berkeley/2010]]

-	[[http://www.epernicus.com/th2 Epernicus]]	+	[http://www.epernicus.com/th2 Epernicus]

Tim Hsiau at 07:03, 20 November 2010

2010-11-20T07:03:52Z

←Older revision		Revision as of 07:03, 20 November 2010
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	[[IGEM:Berkeley/2010]]		[[IGEM:Berkeley/2010]]

-	[[http://www.epernicus.com/th2]]	+	[[http://www.epernicus.com/th2 Epernicus]]

Tim Hsiau: /* Contact Info */

2010-06-20T19:03:03Z

Contact Info

←Older revision		Revision as of 19:03, 20 June 2010
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	[[IGEM:Berkeley/2010]]		[[IGEM:Berkeley/2010]]
		+
		+	[[http://www.epernicus.com/th2]]

Tim Hsiau: /* Contact Info */

2010-06-20T19:00:06Z

Contact Info

←Older revision		Revision as of 19:00, 20 June 2010
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	==Contact Info==		==Contact Info==
-	[[Image:~~TimHsiau~~.jpg\|thumb\|right\|Tim Hsiau]]	+	[[Image:Tim_Hsiau.jpg\|thumb\|right\|Tim Hsiau]]

	*Tim Hsiau		*Tim Hsiau

Tim Hsiau at 18:57, 20 June 2010

2010-06-20T18:57:35Z

←Older revision		Revision as of 18:57, 20 June 2010
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	==Contact Info==		==Contact Info==
-	[[Image:~~OWWEmblem~~.~~png~~\|thumb\|right\|Tim Hsiau ~~(an artistic interpretation)~~]]	+	[[Image:TimHsiau.jpg\|thumb\|right\|Tim Hsiau]]

	*Tim Hsiau		*Tim Hsiau

Tim Hsiau at 18:55, 20 June 2010

2010-06-20T18:55:13Z

←Older revision		Revision as of 18:55, 20 June 2010
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	*Anderson Lab, Berkeley		*Anderson Lab, Berkeley

-	[[~~Berkeley_iGEM10~~]]	+	[[IGEM:Berkeley/2010]]

Tim Hsiau at 20:14, 1 June 2010

2010-06-01T20:14:12Z

←Older revision		Revision as of 20:14, 1 June 2010
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	*Tim Hsiau		*Tim Hsiau
	*Anderson Lab, Berkeley		*Anderson Lab, Berkeley
		+
		+	[[Berkeley_iGEM10]]

Tim Hsiau: /* Molecular Biology tips and tricks */

2010-03-19T08:23:55Z

Molecular Biology tips and tricks

←Older revision		Revision as of 08:23, 19 March 2010
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	*Tim Hsiau		*Tim Hsiau
	*Anderson Lab, Berkeley		*Anderson Lab, Berkeley
-
-
-	~~==Molecular Biology tips and tricks==~~
-	#'''PCR Master Mix'''<br>Make your own PCR master mix that you can freeze and reuse. You can duplicate the Roche FastStart PCR Master mix by simply making a lot of your own master mix, aliquoting it into tubes, and freezing. This mastermix is great for diagnostic colony PCRs, where you can get away with the sloppier Taq polymerase, but also has worked fine with Expand and Phusion in our hands.<br>You can probably apply this approach to other commonly performed reactions as well. Takara sells a DNA Ligation Mighty Mix that's simply a pre-mixed solution of T4 Ligase, ATP, and PEG (optional, helps speed up the ligation).
-	#'''Buy scoops for commonly made media'''<br>Molecular biology labs go through a lot of LB media and, consequently, some poor grad student has the pleasant job of inhaling LB powder dust while measuring out the 25 grams of LB powder that goes into each liter of DI water. You can make media preparation faster and safer by buying premade scoops. We've found that 32cc or 33cc scoops hold 26 grams of LB powder. Scoops can be had by requesting samples from National Measures or buying from http://www.lemelange.com/scoop.htm

Tim Hsiau: /* Molecular Biology tips and tricks */

2010-01-30T04:22:15Z

Molecular Biology tips and tricks

←Older revision		Revision as of 04:22, 30 January 2010
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	==Molecular Biology tips and tricks==		==Molecular Biology tips and tricks==
-	#PCR Master Mix <br>Make your own PCR master mix that you can freeze and reuse. You can duplicate the Roche FastStart PCR Master mix by simply making a lot of your own master mix, aliquoting it into tubes, and freezing. This mastermix is great for diagnostic colony PCRs, where you can get away with the sloppier Taq polymerase, but also has worked fine with Expand and Phusion in our hands.<br>You can probably apply this approach to other commonly performed reactions as well. Takara sells a DNA Ligation Mighty Mix that's simply a pre-mixed solution of T4 Ligase, ATP, and PEG (optional, helps speed up the ligation).	+	#'''PCR Master Mix'''<br>Make your own PCR master mix that you can freeze and reuse. You can duplicate the Roche FastStart PCR Master mix by simply making a lot of your own master mix, aliquoting it into tubes, and freezing. This mastermix is great for diagnostic colony PCRs, where you can get away with the sloppier Taq polymerase, but also has worked fine with Expand and Phusion in our hands.<br>You can probably apply this approach to other commonly performed reactions as well. Takara sells a DNA Ligation Mighty Mix that's simply a pre-mixed solution of T4 Ligase, ATP, and PEG (optional, helps speed up the ligation).
-	#Buy scoops for commonly made media<br>Molecular biology labs go through a lot of LB ~~(or insert your favorite kind here)~~ media and, consequently, some poor grad student has the pleasant job of inhaling LB powder dust while measuring out the 25 grams of LB powder that goes into each liter of DI water. You can make media preparation faster and safer by buying premade scoops. We've found that 32cc or 33cc scoops hold 26 grams of LB powder. Scoops can be had by requesting samples from National Measures or buying from http://www.lemelange.com/scoop.htm	+	#'''Buy scoops for commonly made media'''<br>Molecular biology labs go through a lot of LB media and, consequently, some poor grad student has the pleasant job of inhaling LB powder dust while measuring out the 25 grams of LB powder that goes into each liter of DI water. You can make media preparation faster and safer by buying premade scoops. We've found that 32cc or 33cc scoops hold 26 grams of LB powder. Scoops can be had by requesting samples from National Measures or buying from http://www.lemelange.com/scoop.htm

Tim Hsiau: /* Molecular Biology tips and tricks */

2010-01-30T04:21:35Z

Molecular Biology tips and tricks

←Older revision		Revision as of 04:21, 30 January 2010
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	==Molecular Biology tips and tricks==		==Molecular Biology tips and tricks==
-	#PCR Master Mix <br>Make your own PCR master mix that you can freeze and reuse. You can duplicate the Roche FastStart PCR Master mix by simply making a lot of your own master mix, aliquoting it into tubes, and freezing. This mastermix is great for diagnostic colony PCRs, where you can get away with the sloppier Taq polymerase, but also has worked fine with Expand and Phusion in our hands.	+	#PCR Master Mix <br>Make your own PCR master mix that you can freeze and reuse. You can duplicate the Roche FastStart PCR Master mix by simply making a lot of your own master mix, aliquoting it into tubes, and freezing. This mastermix is great for diagnostic colony PCRs, where you can get away with the sloppier Taq polymerase, but also has worked fine with Expand and Phusion in our hands.<br>You can probably apply this approach to other commonly performed reactions as well. Takara sells a DNA Ligation Mighty Mix that's simply a pre-mixed solution of T4 Ligase, ATP, and PEG (optional, helps speed up the ligation).
-	You can probably apply this approach to other commonly performed reactions as well. Takara sells a DNA Ligation Mighty Mix that's simply a pre-mixed solution of T4 Ligase, ATP, and PEG (optional, helps speed up the ligation).	+
	#Buy scoops for commonly made media<br>Molecular biology labs go through a lot of LB (or insert your favorite kind here) media and, consequently, some poor grad student has the pleasant job of inhaling LB powder dust while measuring out the 25 grams of LB powder that goes into each liter of DI water. You can make media preparation faster and safer by buying premade scoops. We've found that 32cc or 33cc scoops hold 26 grams of LB powder. Scoops can be had by requesting samples from National Measures or buying from http://www.lemelange.com/scoop.htm		#Buy scoops for commonly made media<br>Molecular biology labs go through a lot of LB (or insert your favorite kind here) media and, consequently, some poor grad student has the pleasant job of inhaling LB powder dust while measuring out the 25 grams of LB powder that goes into each liter of DI water. You can make media preparation faster and safer by buying premade scoops. We've found that 32cc or 33cc scoops hold 26 grams of LB powder. Scoops can be had by requesting samples from National Measures or buying from http://www.lemelange.com/scoop.htm