|
|
Line 346: |
Line 346: |
| or the following matrix with the amino acids as a 4-factor categorical with none, glu, arg, glu+arg | | or the following matrix with the amino acids as a 4-factor categorical with none, glu, arg, glu+arg |
|
| |
|
| {| {{table}}
| | |
| | align="center" style="background:#f0f0f0;"|'''buffer #'''
| |
| | align="center" style="background:#f0f0f0;"|'''pH'''
| |
| | align="center" style="background:#f0f0f0;"|'''detergent'''
| |
| | align="center" style="background:#f0f0f0;"|'''amino acids'''
| |
| | align="center" style="background:#f0f0f0;"|'''salt'''
| |
| | align="center" style="background:#f0f0f0;"|'''crowders'''
| |
| | align="center" style="background:#f0f0f0;"|'''divalent cation'''
| |
| | align="center" style="background:#f0f0f0;"|'''GnCl'''
| |
| | align="center" style="background:#f0f0f0;"|'''BSA'''
| |
| | align="center" style="background:#f0f0f0;"|'''Ligand'''
| |
| | align="center" style="background:#f0f0f0;"|'''reducing agent'''
| |
| | align="center" style="background:#f0f0f0;"|'''Amino acid'''
| |
| |-
| |
| | 1||5.5 MES||none||none||low||none||1||0||1||1||none||Arg
| |
| |-
| |
| | 2||5.5 MES||none||none||high||Glycerol||0||1||1||1||TCEP||Glu
| |
| |-
| |
| | 3||5.5 MES||none||Glut||none||none||0||0||0||0||none||Arg+Glu
| |
| |-
| |
| | 4||5.5 MES||none||Glut||low||Glycerol||0||0||0||0||TCEP||none
| |
| |-
| |
| | 5||5.5 MES||TritonX||none||high||none||1||0||0||1||none||Glu
| |
| |-
| |
| | 6||5.5 MES||TritonX||Arg||low||PEG||1||1||1||1||none||Arg+Glu
| |
| |-
| |
| | 7||5.5 MES||TritonX||Arg||low||Glycerol||0||1||0||0||TCEP||Arg
| |
| |-
| |
| | 8||5.5 MES||TritonX||Arg||high||Glycerol||1||1||1||0||none||Arg+Glu
| |
| |-
| |
| | 9||5.5 MES||TritonX||Glut||none||none||1||0||1||0||TCEP||Arg+Glu
| |
| |-
| |
| | 10||5.5 MES||Tween80||none||none||PEG||0||1||1||1||DTT||none
| |
| |-
| |
| | 11||5.5 MES||Tween80||none||none||Glycerol||1||1||0||0||DTT||Arg
| |
| |-
| |
| | 12||5.5 MES||Tween80||Arg||none||PEG||0||0||0||1||TCEP||Arg
| |
| |-
| |
| | 13||5.5 MES||Tween80||Arg||low||Glycerol||0||0||0||0||none||Glu
| |
| |-
| |
| | 14||5.5 MES||Tween80||Arg||high||none||1||1||1||1||DTT||Arg
| |
| |-
| |
| | 15||5.5 MES||CHAPS||none||high||Glycerol||0||0||0||0||DTT||Arg+Glu
| |
| |-
| |
| | 16||5.5 MES||CHAPS||Arg||none||none||1||0||0||1||TCEP||none
| |
| |-
| |
| | 17||5.5 MES||CHAPS||Glut||none||PEG||0||1||0||1||none||Glu
| |
| |-
| |
| | 18||5.5 MES||CHAPS||Glut||low||PEG||1||1||1||0||DTT||Glu
| |
| |-
| |
| | 19||5.5 MES||CHAPS||Glut||high||PEG||1||1||1||0||DTT||none
| |
| |-
| |
| | 20||6.5 MES||none||none||none||none||1||1||0||0||none||Arg
| |
| |-
| |
| | 21||6.5 MES||none||none||low||Glycerol||1||0||0||1||DTT||none
| |
| |-
| |
| | 22||6.5 MES||none||Arg||none||PEG||1||0||0||1||DTT||none
| |
| |-
| |
| | 23||6.5 MES||none||Glut||high||none||0||1||1||0||none||Arg+Glu
| |
| |-
| |
| | 24||6.5 MES||none||Glut||high||PEG||0||1||0||0||TCEP||Arg+Glu
| |
| |-
| |
| | 25||6.5 MES||TritonX||none||low||none||0||1||0||0||TCEP||none
| |
| |-
| |
| | 26||6.5 MES||TritonX||none||high||Glycerol||1||1||1||1||DTT||Arg
| |
| |-
| |
| | 27||6.5 MES||TritonX||Arg||none||none||0||1||0||0||none||Glu
| |
| |-
| |
| | 28||6.5 MES||TritonX||Arg||high||PEG||1||0||1||1||DTT||Arg+Glu
| |
| |-
| |
| | 29||6.5 MES||TritonX||Glut||low||Glycerol||1||0||1||1||none||Arg
| |
| |-
| |
| | 30||6.5 MES||Tween80||Arg||none||none||0||1||1||1||DTT||Arg
| |
| |-
| |
| | 31||6.5 MES||Tween80||Arg||none||Glycerol||0||0||1||1||none||Arg+Glu
| |
| |-
| |
| | 32||6.5 MES||Tween80||Glut||low||PEG||1||1||0||0||TCEP||Glu
| |
| |-
| |
| | 33||6.5 MES||Tween80||Glut||low||Glycerol||1||1||1||1||TCEP||none
| |
| |-
| |
| | 34||6.5 MES||Tween80||Glut||high||Glycerol||0||1||1||1||none||Glu
| |
| |-
| |
| | 35||6.5 MES||CHAPS||none||none||none||0||0||1||0||TCEP||Arg
| |
| |-
| |
| | 36||6.5 MES||CHAPS||none||low||PEG||0||0||1||0||none||Glu
| |
| |-
| |
| | 37||6.5 MES||CHAPS||none||low||Glycerol||1||0||0||0||TCEP||Arg+Glu
| |
| |-
| |
| | 38||6.5 MES||CHAPS||Arg||high||PEG||0||0||0||1||TCEP||none
| |
| |-
| |
| | 39||6.5 MES||CHAPS||Arg||high||PEG||1||0||0||0||DTT||Glu
| |
| |-
| |
| | 40||7.5 HEPES||none||none||high||none||1||0||0||1||TCEP||Glu
| |
| |-
| |
| | 41||7.5 HEPES||none||Arg||none||Glycerol||0||0||1||0||DTT||Glu
| |
| |-
| |
| | 42||7.5 HEPES||none||Glut||none||Glycerol||1||1||1||1||TCEP||Arg+Glu
| |
| |-
| |
| | 43||7.5 HEPES||none||Glut||low||none||0||0||1||1||DTT||none
| |
| |-
| |
| | 44||7.5 HEPES||none||Glut||low||PEG||0||0||0||1||DTT||Arg
| |
| |-
| |
| | 45||7.5 HEPES||TritonX||none||none||PEG||1||1||1||0||DTT||Glu
| |
| |-
| |
| | 46||7.5 HEPES||TritonX||none||low||none||0||1||0||1||DTT||Glu
| |
| |-
| |
| | 47||7.5 HEPES||TritonX||none||high||PEG||0||0||1||1||none||Arg
| |
| |-
| |
| | 48||7.5 HEPES||TritonX||Arg||none||PEG||0||0||1||0||none||none
| |
| |-
| |
| | 49||7.5 HEPES||TritonX||Glut||high||Glycerol||0||0||0||0||TCEP||Glu
| |
| |-
| |
| | 50||7.5 HEPES||Tween80||none||low||PEG||1||0||1||1||TCEP||Arg+Glu
| |
| |-
| |
| | 51||7.5 HEPES||Tween80||none||high||Glycerol||1||0||1||0||TCEP||Arg+Glu
| |
| |-
| |
| | 52||7.5 HEPES||Tween80||Arg||high||none||1||0||0||0||DTT||none
| |
| |-
| |
| | 53||7.5 HEPES||Tween80||Glut||none||PEG||1||1||0||0||TCEP||Arg+Glu
| |
| |-
| |
| | 54||7.5 HEPES||Tween80||Glut||high||none||1||1||0||0||none||Arg
| |
| |-
| |
| | 55||7.5 HEPES||CHAPS||none||none||none||0||1||1||1||none||none
| |
| |-
| |
| | 56||7.5 HEPES||CHAPS||Arg||none||Glycerol||1||1||0||0||none||none
| |
| |-
| |
| | 57||7.5 HEPES||CHAPS||Arg||low||none||0||1||1||0||DTT||Arg+Glu
| |
| |-
| |
| | 58||7.5 HEPES||CHAPS||Arg||low||Glycerol||1||1||0||1||TCEP||Arg
| |
| |-
| |
| | 59||7.5 HEPES||CHAPS||Glut||high||Glycerol||0||1||0||1||none||Arg
| |
| |-
| |
| | 60||8.2 Tris||none||none||none||PEG||0||1||0||0||TCEP||Arg
| |
| |-
| |
| | 61||8.2 Tris||none||none||none||Glycerol||0||1||1||0||none||none
| |
| |-
| |
| | 62||8.2 Tris||none||Arg||high||none||1||1||1||1||TCEP||Glu
| |
| |-
| |
| | 63||8.2 Tris||none||Arg||high||PEG||0||1||0||1||none||Arg+Glu
| |
| |-
| |
| | 64||8.2 Tris||none||Arg||high||PEG||1||0||0||0||DTT||Arg
| |
| |-
| |
| | 65||8.2 Tris||TritonX||none||low||PEG||0||0||0||1||none||Arg+Glu
| |
| |-
| |
| | 66||8.2 Tris||TritonX||Glut||none||PEG||1||1||1||0||TCEP||Arg
| |
| |-
| |
| | 67||8.2 Tris||TritonX||Glut||none||Glycerol||1||0||1||1||TCEP||none
| |
| |-
| |
| | 68||8.2 Tris||TritonX||Glut||low||none||1||1||0||0||DTT||none
| |
| |-
| |
| | 69||8.2 Tris||TritonX||Glut||high||Glycerol||0||0||0||1||DTT||none
| |
| |-
| |
| | 70||8.2 Tris||Tween80||none||low||none||0||0||1||0||DTT||Arg
| |
| |-
| |
| | 71||8.2 Tris||Tween80||none||high||none||0||0||0||0||none||none
| |
| |-
| |
| | 72||8.2 Tris||Tween80||Arg||low||PEG||0||1||1||1||TCEP||Glu
| |
| |-
| |
| | 73||8.2 Tris||Tween80||Arg||high||none||1||0||1||0||TCEP||Arg+Glu
| |
| |-
| |
| | 74||8.2 Tris||CHAPS||none||none||Glycerol||1||1||0||1||DTT||Arg+Glu
| |
| |-
| |
| | 75||8.2 Tris||CHAPS||Arg||low||Glycerol||0||1||1||0||DTT||Glu
| |
| |-
| |
| | 76||8.2 Tris||CHAPS||Glut||none||none||1||0||1||1||none||Glu
| |
| |-
| |
| | 77||8.2 Tris||CHAPS||Glut||low||Glycerol||1||0||0||1||none||Arg
| |
| |-
| |
| | 78||9.2 CHES||none||none||none||Glycerol||1||0||1||0||DTT||Glu
| |
| |-
| |
| | 79||9.2 CHES||none||Arg||low||none||0||1||0||1||TCEP||Arg+Glu
| |
| |-
| |
| | 80||9.2 CHES||none||Arg||low||PEG||1||0||1||0||none||Arg
| |
| |-
| |
| | 81||9.2 CHES||none||Arg||low||Glycerol||1||1||1||0||none||none
| |
| |-
| |
| | 82||9.2 CHES||none||Glut||high||none||1||1||1||1||DTT||Glu
| |
| |-
| |
| | 83||9.2 CHES||TritonX||none||high||Glycerol||0||1||1||0||TCEP||none
| |
| |-
| |
| | 84||9.2 CHES||TritonX||Arg||none||none||1||0||0||1||TCEP||Glu
| |
| |-
| |
| | 85||9.2 CHES||TritonX||Arg||high||Glycerol||0||1||0||0||DTT||Arg
| |
| |-
| |
| | 86||9.2 CHES||TritonX||Glut||none||PEG||0||1||0||1||DTT||Arg+Glu
| |
| |-
| |
| | 87||9.2 CHES||Tween80||none||low||PEG||1||1||0||1||none||none
| |
| |-
| |
| | 88||9.2 CHES||Tween80||none||high||PEG||1||1||0||0||none||Glu
| |
| |-
| |
| | 89||9.2 CHES||Tween80||Arg||none||Glycerol||1||0||0||1||none||Glu
| |
| |-
| |
| | 90||9.2 CHES||Tween80||Glut||none||Glycerol||0||0||0||1||DTT||Arg+Glu
| |
| |-
| |
| | 91||9.2 CHES||Tween80||Glut||low||none||0||0||1||0||DTT||Arg+Glu
| |
| |-
| |
| | 92||9.2 CHES||CHAPS||none||low||none||1||1||0||1||DTT||Arg+Glu
| |
| |-
| |
| | 93||9.2 CHES||CHAPS||none||high||PEG||0||0||1||1||TCEP||Arg
| |
| |-
| |
| | 94||9.2 CHES||CHAPS||Arg||high||none||0||1||1||1||TCEP||none
| |
| |-
| |
| | 95||9.2 CHES||CHAPS||Glut||none||none||0||0||1||0||TCEP||Arg
| |
| |-
| |
| | 96||9.2 CHES||CHAPS||Glut||high||PEG||1||0||1||0||none||none
| |
| |-
| |
| |
| |
| |}
| |
|
| |
|
| ==References== | | ==References== |