User:Tk/Notebook/MF-xfm/2008/04/14: Difference between revisions
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(Autocreate 2008/04/14 Entry for User:Tk/Notebook/MF-xfm) |
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== | ==Transformations== | ||
* | * Some small colonies of a few of the older plates | ||
** likely junk | |||
==PCR of transposons== | |||
* 20 ul reactions | |||
* 0.6 ul ME0 primer | |||
* cycle 95/5 min, (95/20s, 55/20s, 72/3.0 min) x 32 | |||
* wells: | |||
** 83 ng/ul cut PCR'd transposon | |||
** transposome | |||
** cells from culture B of initial transformations (streaked plate for growth test) | |||
==Setup of cultures for tomorrow== | |||
* 50 ml cultures infected with | |||
** 5 ml | |||
** 0.5 ml | |||
** 50 ul | |||
** 5 ul | |||
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Revision as of 21:49, 19 June 2008
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Transformations
PCR of transposons
Setup of cultures for tomorrow
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