User:Tk/Notebook/MF-xfm/2008/04/22: Difference between revisions
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(Autocreate 2008/04/22 Entry for User:Tk/Notebook/MF-xfm) |
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== | ==Construction plasmid PCR== | ||
* | * 100 ul final | ||
* 50 ul Phusion mastermix | |||
* 1.5 ul suffix-FB | |||
* 1.5 ul prefix-RB | |||
* 0.5 ul of plasmid (pSB1AC3, 1AK3, 1AT3) | |||
* 46.5 ul water | |||
* Cycle 98/30s 32x(98/20s 55/20s 72/2min) 72/10min | |||
==Transformation results== | |||
* Picked 40 colonies off of 4/20 transformations | |||
* +10 worked best, 0 worked well, -5 few, -10 none, +15 few | |||
* replated 0,-5 saved cultures | |||
==Construction plasmid distribution== | |||
* Each team gets 300 ul of each plasmid | |||
* Concentration is 10 ng/ul | |||
* Each team needs 3 ug of plasmid | |||
* With 150 teams, we need 450 ug of each plasmid | |||
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Revision as of 21:54, 19 June 2008
 MF-xfm
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Construction plasmid PCR
Transformation results
Construction plasmid distribution
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