User:Tk/Notebook/MF/2008/10/28: Difference between revisions
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===Re-do plasmid PCRs with a higher annealing temperature=== | |||
===PCR purify J70023, 23-F2, 24, 25, 26=== | |||
Latest revision as of 17:02, 28 October 2008
 Mesoplasma florum simplification
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Biobrick MF componentsPlasmid backbones
J70007, recT gene coding regiongtttcttcgaattcgcggccgcttctagatgaataataaaattgaattaatattaaataattc,recT-F gtttcttctactagtagcggccgctgcagttattaatcagaaaacatttcattaacagc,recT-R gaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagc,recT-mut-F gctgttattaataattattgcttggtcatgttgataggcttcttgcatttc,recT-mut-R
858 bp J70023, MF plasmid originGTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 Template: Spiroplasma citri genomic DNA (Renaudin) 1878 bp J70024, tetM gene coding regionGTTTCTTCGAATTCGCGGCCGCTtctagatgaaaatcataaatatcgg,J00024-F GTTTCTTCCTGCAGCGGCCGCTactagtattattatgtgattttgttg,J00024-R Template: pTT01 plasmid DNA 1920 bp J70025, tetM promoter region (pBOT1)GTTTCTTCGAATTCGCGGCCGCTTCTAGAGggccagtctacatgtactc,J00025-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAttcctccattcaaaaacccaa,J00025-R Template: pTT01 345 bp long J70026, cat resistance cassetteGTTTCTTCGAATTCGCGGCCGCTTCTAGAGaatagttacccttattatcaag,J70026-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAtctggagctgtaatataaaaacc,J70026-R Template: pKV104 plasmid (Krause) 1030 bp PCR results
Re-do plasmid PCRs with a higher annealing temperaturePCR purify J70023, 23-F2, 24, 25, 26 | |
