User:Tk/Notebook/MF/2008/10/31: Difference between revisions

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* J70007 into pSB1AK3
* J70007 into pSB1AK3


==Backbone construction==
* PCR, column cleanup (100 ul volume, elute to 50 ul, digest at 100 ul)
* digest, heat kill, add 150 ul beads, add 500 mM salt, 1 mM EDTA, volume at 276 ul)
* bind, remove beads
* dilute with 5x PB (1.4 ml), column bind total of 1.7 ml, 3x column loading
* elute




Latest revision as of 12:35, 31 October 2008

Mesoplasma florum simplification <html><img src="/images/9/94/Report.png" border="0" /></html> Main Page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Part cloning

  • J70023, J70023-F2 into pSB1AK3
  • J70024, J70025 into pSB1AC3
  • J70026 into pSB1AK3
  • J70007 into pSB1AK3


Backbone construction

  • PCR, column cleanup (100 ul volume, elute to 50 ul, digest at 100 ul)
  • digest, heat kill, add 150 ul beads, add 500 mM salt, 1 mM EDTA, volume at 276 ul)
  • bind, remove beads
  • dilute with 5x PB (1.4 ml), column bind total of 1.7 ml, 3x column loading
  • elute



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