User:Tk/Notebook/MF/2008/11/05: Difference between revisions
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(Autocreate 2008/11/05 Entry for User:Tk/Notebook/MF) |
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== | ==Vector backbone== | ||
* | * gave up on biotin purification | ||
* PCR cleanup after PCR with plain primers (62 C anneal) 100 ul rxn | |||
* elute 2x with 40 ul in EB | |||
* digestion in 100 ul with E/P 2 hr / 20 min heat kill | |||
* PCR cleanup, elute 2x with 40 ul | |||
* yields 1AC3: 48 1AK3: 69 1AT3: 57 ng/ul | |||
==Transformation results== | |||
* two colonies on the AC plate, none on others. | |||
* control normal at 4e7 efficiency | |||
Revision as of 09:40, 5 November 2008
 Mesoplasma florum simplification
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Vector backbone
Transformation results
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