User:Tk/Notebook/MF/2009/03/21: Difference between revisions
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==RNAse H genes== | ==RNAse H genes; Genomic DNA prep for Illumina sequencing== | ||
Kochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are: | Kochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are: | ||
* -I (with dsRHbd) is Mfl475, and is apparently essential | * -I (with dsRHbd) is Mfl475, and is apparently essential | ||
* - | * -III is Mfl537, and has been knocked out several times. | ||
Made new 1161 plates - no antibiotic | Made new 1161 plates - no antibiotic | ||
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Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing. | Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing. | ||
Prepare seed cultures of L1, GF1, | Prepare seed cultures of L1, GF1, PP1, W12, M. entomophilum | ||
Latest revision as of 12:10, 8 May 2012
 Mesoplasma florum simplification
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RNAse H genes; Genomic DNA prep for Illumina sequencingKochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are:
Made new 1161 plates - no antibiotic Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing. Prepare seed cultures of L1, GF1, PP1, W12, M. entomophilum
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