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Revision as of 19:53, 19 July 2009

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid

Relevant properties or genotype

Source


Strains

E. coli DH5

F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169

[a]

E. coli M15 [pREP4]

F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR

[a]

Pseudomonas putida KT2440

type strain

[b]

Synechocystis sp. PCC 6803

type strain

[c]

Plasmids

pCAR16

-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR

[27]

pJF119EH

cloning vector, tac-promoter, IPTG inducible, AmpR

[25]

pJF119N

cloning vector, RBS, tac-promoter, IPTG inducible, AmpR

this study

pAW229

cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR

[26]

pQE31-VTE1

tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR

[18a]

pCAS2JF

1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH

this study

pCAS8

1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N

this study

pCAS11

0.9 kb BglII/BamHI PCR fragment crtE in pCAS8

this study

pCAS12

1.0 kb BglII/BamHI PCR fragment hpt in pCAS11

this study

pCAS15

1.2 kb NdeI/BamHI PCR fragment hpd in pAW229

this study

pCAS18

1.2 kb BglII/BamHI PCR fragment hpd in pCAS11

this study

pCAS19

1.0 kb NdeI/BamHI PCR fragment hpt in pAW229

this study

pCAS23

1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N

this study

pCAS24

0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23

this study

pCAS27

1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229

this study

pCAS29

1.2 kb BglII/BamHI PCR fragment hpd in pCAS18

this study

pCAS30

0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N

this study

pCAS37

1.2 kb BglII/BamHI PCR fragment hpd in pCAS24

this study

pCAS47

1.3 kb BglII PCR fragment vte1 in pCAS29

this study


   [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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