User:Tom Adie/Sandbox/1

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(New page: <html><TABLE WIDTH="80%" ><TR ><TD ><A NAME="TBL1" >&nbsp;</A ></TD ></TR ><TR ><TD ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR ><TR VALIGN...)
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<td ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR >
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<table >
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<tr >
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>Table 1. </B
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>Strains and plasmids used in this work.</B
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<p>Strain or plasmid</TH >
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<th ALIGN="left" VALIGN="TOP" >
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<p>Relevant properties or genotype</TH >
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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<p>Strains</NOBR ></TD >
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<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD >
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<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR >
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<td ALIGN="left" VALIGN="TOP" >
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><P>Strain or plasmid</TH
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<p><I >E. coli</I > DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD >
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><P>Relevant properties or genotype</TH
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<p>F<SUP >-</SUP >, <I ><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I >80d, <I >lacZ</I ><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I >endA</I >1, <I >recA</I >1, <I >hsdR</I >17(r<SUB >K</SUB ><SUP >-</SUP >m<SUB >K</SUB ><SUP >-</SUP >), <I >supE</I >44, <I >thi</I >-1, <I >gyrA</I >96, <I >relA</I >1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I >lacZYA-argF</I >)U169</TD >
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<p><I >E. coli</I > M15 [pREP4]</TD >
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COLSPAN="3"
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<p>F<SUP >-</SUP >, <I >lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I >-1, <I >lac<SUP >-</SUP ></I >, <I >mtl<SUP >-</SUP ></I >, <I >recA<SUP >+</SUP ></I > , Km<SUP >R</SUP ></TD >
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><HR
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size="1"></TD
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<p>[a]</NOBR ></TD ></TR >
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<tr >
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<td ALIGN="left" VALIGN="TOP" >
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<p><I >Pseudomonas putida</I > KT2440</TD >
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><NOBR
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<td ALIGN="left" VALIGN="TOP" >
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><P>Strains</NOBR
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></TD
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<p>type strain</TD >
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><TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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<p>[b]</NOBR ></TD ></TR >
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<tr >
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></NOBR
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></TD
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><TD
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<p><I >Synechocystis</I > sp. PCC 6803</TD >
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
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><NOBR
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<p>type strain</TD >
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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></TD
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<p>[c]</NOBR ></TD ></TR >
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<tr >
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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<p>Plasmids</NOBR ></TD >
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><P><I
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<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD >
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>E. coli</I
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<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR >
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> DH5<IMG
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SRC="/giflibrary/12/alpha.gif"
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BORDER="0"></TD
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<p>pCAR16</NOBR ></TD >
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><P>F<SUP
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<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I >Pantoea ananatis</I > in pUC19, Amp<SUP >R</SUP ></TD >
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>-</SUP
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>, <I
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<p>[<A HREF="#BIB27" >27</A >]</NOBR ></TD ></TR >
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SRC="/giflibrary/12/phgr.gif"
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BORDER="0"
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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ALIGN="ABSBOTTOM"></I
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>80d, <I
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<p>pJF119EH</NOBR ></TD >
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>lacZ</I
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<td ALIGN="left" VALIGN="TOP" >
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><IMG
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-
SRC="/giflibrary/12/Delta.gif"
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<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD >
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BORDER="0">M15, <I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>endA</I
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>1, <I
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<p>[<A HREF="#BIB25" >25</A >]</NOBR ></TD ></TR >
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>recA</I
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<tr >
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>1, <I
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>hsdR</I
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>17(r<SUB
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<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR ></TD >
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>K</SUB
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<td ALIGN="left" VALIGN="TOP" >
-
><SUP
+
 
-
>-</SUP
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<p>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD >
-
>m<SUB
+
<td ALIGN="left" VALIGN="TOP" >
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>K</SUB
+
 
-
><SUP
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<p>this study</TD ></TR >
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>), <I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>supE</I
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>44, <I
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<p>pAW229</NOBR ></TD >
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>thi</I
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<td ALIGN="left" VALIGN="TOP" >
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>-1, <I
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>gyrA</I
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<p>cloning vector, RBS, <I >rha</I >-promoter, <FONT SIZE="-1" >L</FONT >-rhamnose inducible, Cm<SUP >R</SUP ></TD >
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>96, <I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>relA</I
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>1, <IMG
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<p>[<A HREF="#BIB26" >26</A >]</NOBR ></TD ></TR >
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SRC="/giflibrary/12/Delta.gif"
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<tr >
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BORDER="0">(<I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>lacZYA-argF</I
+
 
-
>)U169</TD
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<p>pQE31-VTE1</NOBR ></TD >
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><TD
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<td ALIGN="left" VALIGN="TOP" >
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ALIGN="left"
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VALIGN="TOP"
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<p>tocopherol-cyclase (vte1) from <I >Arabidopsis thaliana</I > in pQE31, IPTG inducible, Amp<SUP >R</SUP ></TD >
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><NOBR
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><P>[a]</NOBR
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-
></TD
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<p>[<A HREF="#BIB18A" >18a</A >]</NOBR ></TD ></TR >
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></TR
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<tr >
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><TD
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<p>pCAS2JF</NOBR ></TD >
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP" >
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><P><I
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>E. coli</I
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<p>1.2 kb EcoRI/BamHI PCR fragment <I >hpd</I > in pJF119EH</TD >
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> M15 [pREP4]</TD
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<td ALIGN="left" VALIGN="TOP" >
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><TD
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-
ALIGN="left"
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<p>this study</TD ></TR >
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VALIGN="TOP"
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<tr >
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><P>F<SUP
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>-</SUP
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>, <I
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<p>pCAS8</NOBR ></TD >
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>lacZ<IMG
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<td ALIGN="left" VALIGN="TOP" >
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SRC="/giflibrary/12/Delta.gif"
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BORDER="0">M15, thi</I
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<p>1.1 kb NdeI/BamHI PCR fragment <I >ggh</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
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>-1, <I
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<td ALIGN="left" VALIGN="TOP" >
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>lac<SUP
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-
>-</SUP
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<p>this study</TD ></TR >
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></I
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>, <I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>mtl<SUP
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>-</SUP
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<p>pCAS11</NOBR ></TD >
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></I
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<td ALIGN="left" VALIGN="TOP" >
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>, <I
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>recA<SUP
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<p>0.9 kb BglII/BamHI PCR fragment <I >crtE</I > in pCAS8</TD >
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>+</SUP
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<td ALIGN="left" VALIGN="TOP" >
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></I
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> , Km<SUP
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<p>this study</TD ></TR >
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>R</SUP
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<tr >
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></TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><TD
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<p>pCAS12</NOBR ></TD >
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP" >
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><NOBR
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><P>[a]</NOBR
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<p>1.0 kb BglII/BamHI PCR fragment <I >hpt</I > in pCAS11</TD >
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></TD
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<td ALIGN="left" VALIGN="TOP" >
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></TR
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-
><TR
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<p>this study</TD ></TR >
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><TD
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<tr >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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VALIGN="TOP"
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><P><I
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<p>pCAS15</NOBR ></TD >
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>Pseudomonas putida</I
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<td ALIGN="left" VALIGN="TOP" >
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> KT2440</TD
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-
><TD
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<p>1.2 kb NdeI/BamHI PCR fragment <I >hpd</I > in pAW229</TD >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
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-
><P>type strain</TD
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<p>this study</TD ></TR >
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><TD
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<tr >
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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<p>pCAS18</NOBR ></TD >
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><P>[b]</NOBR
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<td ALIGN="left" VALIGN="TOP" >
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></TD
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></TR
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<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS11</TD >
-
><TR
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<td ALIGN="left" VALIGN="TOP" >
-
><TD
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-
ALIGN="left"
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<p>this study</TD ></TR >
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VALIGN="TOP"
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<tr >
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><P><I
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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>Synechocystis</I
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> sp. PCC 6803</TD
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<p>pCAS19</NOBR ></TD >
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><TD
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<td ALIGN="left" VALIGN="TOP" >
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ALIGN="left"
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-
VALIGN="TOP"
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<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pAW229</TD >
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><P>type strain</TD
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<td ALIGN="left" VALIGN="TOP" >
-
><TD
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ALIGN="left"
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<p>this study</TD ></TR >
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VALIGN="TOP"
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<tr >
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><NOBR
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><P>[c]</NOBR
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<p>pCAS23</NOBR ></TD >
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></TR
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<td ALIGN="left" VALIGN="TOP" >
-
><TR
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-
><TD
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<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
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-
><NOBR
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<p>this study</TD ></TR >
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><P>Plasmids</NOBR
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<tr >
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></TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><TD
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ALIGN="left"
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<p>pCAS24</NOBR ></TD >
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP" >
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><NOBR
+
 
-
></NOBR
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<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pCAS23</TD >
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></TD
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<td ALIGN="left" VALIGN="TOP" >
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><TD
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-
ALIGN="left"
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<p>this study</TD ></TR >
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VALIGN="TOP"
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<tr >
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><NOBR
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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></NOBR
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></TD
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<p>pCAS27</NOBR ></TD >
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></TR
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<td ALIGN="left" VALIGN="TOP" >
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><TR
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><TD
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<p>1.1 kb NdeI/BamHI PCR fragment <I >cyc-sy</I > in pAW229</TD >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
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-
><NOBR
+
<p>this study</TD ></TR >
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><P>pCAR16</NOBR
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<tr >
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></TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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><TD
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ALIGN="left"
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<p>pCAS29</NOBR ></TD >
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP" >
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><P><IMG
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SRC="/giflibrary/12/beta.gif"
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<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS18</TD >
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BORDER="0"
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<td ALIGN="left" VALIGN="TOP" >
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ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I
+
 
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>Pantoea ananatis</I
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<p>this study</TD ></TR >
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> in pUC19, Amp<SUP
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<tr >
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>R</SUP
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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></TD
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><TD
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
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-
><NOBR
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<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
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><P>[<A
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<td ALIGN="left" VALIGN="TOP" >
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HREF="#BIB27"
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>27</A
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<p>this study</TD ></TR >
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>]</NOBR
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<tr >
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></TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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></TR
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><TR
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<p>pCAS37</NOBR ></TD >
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><TD
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<td ALIGN="left" VALIGN="TOP" >
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ALIGN="left"
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VALIGN="TOP"
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<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS24</TD >
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><NOBR
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<td ALIGN="left" VALIGN="TOP" >
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><P>pJF119EH</NOBR
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></TD
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<p>this study</TD ></TR >
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><TD
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<tr >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
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VALIGN="TOP"
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><P>cloning vector, tac-promoter, IPTG inducible, Amp<SUP
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<p>pCAS47</NOBR ></TD >
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>R</SUP
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<td ALIGN="left" VALIGN="TOP" >
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></TD
+
 
-
><TD
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<p>1.3 kb BglII PCR fragment <I >vte1</I > in pCAS29</TD >
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP" >
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VALIGN="TOP"
+
 
-
><NOBR
+
<p>this study</TD ></TR ></TABLE ></TD ></TR >
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><P>[<A
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<tr VALIGN="BOTTOM" >
-
HREF="#BIB25"
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<td >
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>25</A
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<hr size="2" noshade="1"></TD ></TR ></TABLE ></CENTER ><CENTER >
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>]</NOBR
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<table WIDTH="80%" >
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></TD
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<tr >
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></TR
+
<td ><FONT SIZE="-1" >&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).
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><TR
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<br></FONT ></TD ></TR ></TABLE >
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</html>
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><NOBR
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><P>pJF119<IMG
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+
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></TD
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><TD
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VALIGN="TOP"
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><P>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP
+
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>R</SUP
+
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></TD
+
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><TD
+
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VALIGN="TOP"
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><P>this study</TD
+
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></TR
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+
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+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pAW229</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>cloning vector, RBS, <I
+
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>rha</I
+
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>-promoter, <FONT
+
-
SIZE="-1"
+
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>L</FONT
+
-
>-rhamnose inducible, Cm<SUP
+
-
>R</SUP
+
-
></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
-
><NOBR
+
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><P>[<A
+
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HREF="#BIB26"
+
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>26</A
+
-
>]</NOBR
+
-
></TD
+
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></TR
+
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><TR
+
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><TD
+
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+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pQE31-VTE1</NOBR
+
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></TD
+
-
><TD
+
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><P>tocopherol-cyclase (vte1) from <I
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> in pQE31, IPTG inducible, Amp<SUP
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><TR
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></TD
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><P>1.2 kb EcoRI/BamHI PCR fragment <I
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>hpd</I
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> in pJF119EH</TD
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></TD
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><P>1.1 kb NdeI/BamHI PCR fragment <I
+
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>ggh</I
+
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> in pJF119<IMG
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SRC="/giflibrary/12/Delta.gif"
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BORDER="0">N</TD
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><TD
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ALIGN="left"
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VALIGN="TOP"
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></TD
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ALIGN="left"
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VALIGN="TOP"
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><P>0.9 kb BglII/BamHI PCR fragment <I
+
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>crtE</I
+
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> in pCAS8</TD
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><P>this study</TD
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></TR
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><TR
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><TD
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><P>pCAS12</NOBR
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></TD
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
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><P>1.0 kb BglII/BamHI PCR fragment <I
+
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>hpt</I
+
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> in pCAS11</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
+
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
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><P>pCAS15</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.2 kb NdeI/BamHI PCR fragment <I
+
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>hpd</I
+
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> in pAW229</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
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><P>this study</TD
+
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></TR
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><TR
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><TD
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ALIGN="left"
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><NOBR
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><P>pCAS18</NOBR
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.2 kb BglII/BamHI PCR fragment <I
+
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>hpd</I
+
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> in pCAS11</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
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><P>pCAS19</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.0 kb NdeI/BamHI PCR fragment <I
+
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>hpt</I
+
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> in pAW229</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
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><P>pCAS23</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.0 kb NdeI/BamHI PCR fragment <I
+
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>hpt</I
+
-
> in pJF119<IMG
+
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SRC="/giflibrary/12/Delta.gif"
+
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BORDER="0">N</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
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><P>pCAS24</NOBR
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></TD
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><TD
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>0.9 kb NdeI/BamHI PCR fragment <I
+
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>crtE</I
+
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> in pCAS23</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
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><P>pCAS27</NOBR
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></TD
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><TD
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ALIGN="left"
+
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VALIGN="TOP"
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><P>1.1 kb NdeI/BamHI PCR fragment <I
+
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>cyc-sy</I
+
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> in pAW229</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
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><TD
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></TD
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><TD
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ALIGN="left"
+
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VALIGN="TOP"
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><P>1.2 kb BglII/BamHI PCR fragment <I
+
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>hpd</I
+
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> in pCAS18</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS30</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>0.9 kb NdeI/BamHI PCR fragment <I
+
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>crtE</I
+
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> in pJF119<IMG
+
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SRC="/giflibrary/12/Delta.gif"
+
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BORDER="0">N</TD
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
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><TD
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VALIGN="TOP"
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><P>pCAS37</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.2 kb BglII/BamHI PCR fragment <I
+
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>hpd</I
+
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> in pCAS24</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
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+
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><P>pCAS47</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.3 kb BglII PCR fragment <I
+
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>vte1</I
+
-
> in pCAS29</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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>&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).<BR></FONT
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Revision as of 21:01, 19 July 2009

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid

Relevant properties or genotype

Source


Strains

E. coli DH5

F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169

[a]

E. coli M15 [pREP4]

F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR

[a]

Pseudomonas putida KT2440

type strain

[b]

Synechocystis sp. PCC 6803

type strain

[c]

Plasmids

pCAR16

-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR

[27]

pJF119EH

cloning vector, tac-promoter, IPTG inducible, AmpR

[25]

pJF119N

cloning vector, RBS, tac-promoter, IPTG inducible, AmpR

this study

pAW229

cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR

[26]

pQE31-VTE1

tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR

[18a]

pCAS2JF

1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH

this study

pCAS8

1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N

this study

pCAS11

0.9 kb BglII/BamHI PCR fragment crtE in pCAS8

this study

pCAS12

1.0 kb BglII/BamHI PCR fragment hpt in pCAS11

this study

pCAS15

1.2 kb NdeI/BamHI PCR fragment hpd in pAW229

this study

pCAS18

1.2 kb BglII/BamHI PCR fragment hpd in pCAS11

this study

pCAS19

1.0 kb NdeI/BamHI PCR fragment hpt in pAW229

this study

pCAS23

1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N

this study

pCAS24

0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23

this study

pCAS27

1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229

this study

pCAS29

1.2 kb BglII/BamHI PCR fragment hpd in pCAS18

this study

pCAS30

0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N

this study

pCAS37

1.2 kb BglII/BamHI PCR fragment hpd in pCAS24

this study

pCAS47

1.3 kb BglII PCR fragment vte1 in pCAS29

this study


   [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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