User:Torsten Waldminghaus/Notebook/dnaC2 temperature shift experiment

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Revision as of 06:06, 12 February 2009 by Torsten Waldminghaus (talk | contribs) (New page: ==Principle== The ''dnaC2'' mutation in ''E. coli'' can be used as tool to synchronize DNA replication. The DnaC protein is needed for initiation of replication. Temperal inactivation via ...)
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Principle

The dnaC2 mutation in E. coli can be used as tool to synchronize DNA replication. The DnaC protein is needed for initiation of replication. Temperal inactivation via temperature shift of the ts mutant DnaC2 leeds to stop of initiation. Shift back to lower temperature leads to restart of replication.

Protocol

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