User:Torsten Waldminghaus/Strains
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(Difference between revisions)
| Line 162: | Line 162: | ||
|aroA::GATCcluster | |aroA::GATCcluster | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx22 | + | |Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta |
|- | |- | ||
! TWx28 | ! TWx28 | ||
| Line 168: | Line 168: | ||
|ter::GATCcluster (1) | |ter::GATCcluster (1) | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx23 | + | |Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
|- | |- | ||
! TWx29 | ! TWx29 | ||
| Line 174: | Line 174: | ||
|ter::GATCcluster (2) | |ter::GATCcluster (2) | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx23 | + | |Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
|- | |- | ||
! TWx30 | ! TWx30 | ||
| Line 180: | Line 180: | ||
|srlA::GATCcluster | |srlA::GATCcluster | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx25 | + | |Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta |
|- | |- | ||
! TWx31 | ! TWx31 | ||
| Line 186: | Line 186: | ||
|hisA:GATCcluster | |hisA:GATCcluster | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx24 | + | |Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta |
|- | |- | ||
! TWx32 | ! TWx32 | ||
| Line 198: | Line 198: | ||
|ter::GATCcluster (ter1) | |ter::GATCcluster (ter1) | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx23 | + | |Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
|- | |- | ||
! TWx34 | ! TWx34 | ||
| Line 204: | Line 204: | ||
|ter::GATCcluster (ter2) | |ter::GATCcluster (ter2) | ||
|cm | |cm | ||
| - | |Transfered to MG1655 by P1 transduction from TWx23 | + | |Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
|- | |- | ||
! TWx35 | ! TWx35 | ||
Revision as of 07:04, 28 January 2010
| Strain | Parental strain | Characteristics | AB resistence | Reference |
|---|---|---|---|---|
| TWx1 | DH5α | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | no | |
| TWx2 | DH5α | pFH2102 | amp | |
| TWx3 | MG1655 | ? | no | |
| TWx4 | CM735 | metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | no | [1] |
| TWx5 | DH5α | pGEM-pyrB | amp | |
| TWx6 | DH5α | pFH2102-pyrB (=ppyrB) | amp | |
| TWx7 | SG2221T | pFH2102 | amp | [2] |
| TWx8 | MG1655 | pyrB::Tn5 | kan | Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain) |
| TWx9 | TW8 | pyrB::Tn5 ppyrB | kan, amp | |
| TWx10 | TW8 | pyrB::Tn5 pFH2102 (empty vector) | kan, amp | |
| TWx11 | TW3 (MG1655) | ppyrB | kan, amp | |
| TWx12 | TW3 (MG1655) | pFH2102 (empty vector) | kan, amp | |
| TWx13 | MG1655 | EBO193 (ΔseqA) (box T24) | no | |
| TWx14 | DH5α | pET3a-Pfu | amp | strain to overproduce and purify Pfu DNA polymerase |
| TWx15 | DH5α | pTaq | amp | strain to overproduce and purify Taq DNA polymerase |
| TWx16 | KS0003 | MG1655 dam16 pGATC | km, cm | |
| TWx17 | MG1655 | pGATC | cm | |
| TWx18 | ALO1826 (box R41) | amp | ||
| TWx19 | ALO1829 (box R43) | amp, cm | ||
| TWx20 | MG1655 | KS0003 (dam16) (box O33) | km | |
| TWx21 | DY330 | proAB::GATCcluster | cm | |
| TWx22 | DY330 | aroA::GATCcluster | cm | |
| TWx23 | DY330 | ter::GATCcluster | cm | |
| TWx24 | DY330 | hisA::GATCcluster | cm | |
| TWx25 | DY330 | srlA::GATCcluster | cm | |
| TWx26 | MG1655 | proAB::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta |
| TWx27 | MG1655 | aroA::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta |
| TWx28 | MG1655 | ter::GATCcluster (1) | cm | Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx29 | MG1655 | ter::GATCcluster (2) | cm | Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx30 | MG1655 | srlA::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta |
| TWx31 | MG1655 | hisA:GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta |
| TWx32 | CM735 | NK9386 | no | strain with IPTG inducible stick flagella for baby cell column [3] |
| TWx33 | MG1655 | ter::GATCcluster (ter1) | cm | Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx34 | MG1655 | ter::GATCcluster (ter2) | cm | Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx35 | MG1655 | ALO1739 (arok17::cat/pMS2) | cm, km | strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4] |
| TWx36 | GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51) | km | The prototype dam-16::Kan deletion strain [5] http://users.umassmed.edu/martin.marinus/dstrains.html | |
| TWx37 | GM3819 | pMQ430/GM3819 | km, cm | pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6] http://users.umassmed.edu/martin.marinus/plasmid.html |
- Hoskins JR, Singh SK, Maurizi MR, and Wickner S. . pmid:10922051.
- Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. . pmid:15978072.
- Løbner-Olesen A, Marinus MG, and Hansen FG. . pmid:12682301.
- Parker B and Marinus MG. . pmid:2854098.
- Calmann MA and Marinus MG. . pmid:12897023.
- Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. . pmid:6094968.
