User:Torsten Waldminghaus/Strains

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|TWX49 (KS9921)
|TWX49 (KS9921)
|ter::GATC-cluster
|ter::GATC-cluster
 +
|cm
|Transfered by P1 transduction from TWx23
|Transfered by P1 transduction from TWx23
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|
 
|-
|-
! TWx51
! TWx51
|TWX50  
|TWX50  
|ter::GATC-cluster; pIO4  
|ter::GATC-cluster; pIO4  
 +
|cm, amp
|seqA4 plasmid, IPTG inducible
|seqA4 plasmid, IPTG inducible
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|
 
|-
|-
|}
|}

Revision as of 08:34, 20 September 2011

Strain Parental strain Characteristics AB resistence Reference
TWx1 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 no
TWx2 DH5α pFH2102 amp
TWx3 MG1655 ? no
TWx4 CM735 metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - no [1]
TWx5 DH5α pGEM-pyrB amp
TWx6 DH5α pFH2102-pyrB (=ppyrB) amp
TWx7 SG2221T pFH2102 amp [2]
TWx8 MG1655 pyrB::Tn5 kan Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain)
TWx9 TW8 pyrB::Tn5 ppyrB kan, amp
TWx10 TW8 pyrB::Tn5 pFH2102 (empty vector) kan, amp
TWx11 TW3 (MG1655) ppyrB amp
TWx12 TW3 (MG1655) pFH2102 (empty vector) amp
TWx13 CM735 EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP no
TWx14 DH5α pET3a-Pfu amp strain to overproduce and purify Pfu DNA polymerase
TWx15 DH5α pTaq amp strain to overproduce and purify Taq DNA polymerase
TWx16 KS0003 MG1655 dam16 pGATC km, cm
TWx17 MG1655 pGATC cm
TWx18 ALO1826 (box R41) amp
TWx19 ALO1829 (box R43) amp, cm
TWx20 MG1655 KS0003 (dam16) (box O33) km
TWx21 DY330 proAB::GATCcluster cm
TWx22 DY330 aroA::GATCcluster cm
TWx23 DY330 ter::GATCcluster cm
TWx24 DY330 hisA::GATCcluster cm
TWx25 DY330 srlA::GATCcluster cm
TWx26 MG1655 proAB::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta
TWx27 MG1655 aroA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta
TWx28 MG1655 ter::GATCcluster (1) cm Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx29 MG1655 ter::GATCcluster (2) cm Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx30 MG1655 srlA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta
TWx31 MG1655 hisA:GATCcluster cm Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta
TWx32 CM735 NK9386 Ptac::fliCst no strain with IPTG inducible stick flagella for baby cell column [3]
TWx33 MG1655 ter::GATCcluster (ter1) cm Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx34 MG1655 ter::GATCcluster (ter2) cm Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx35 MG1655 ALO1739 (arok17::cat/pMS2) cm, km strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4]
TWx36 GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51) km The prototype dam-16::Kan deletion strain [5] http://users.umassmed.edu/martin.marinus/dstrains.html
TWx37 GM3819 pMQ430/GM3819 km, amp pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6] http://users.umassmed.edu/martin.marinus/plasmid.html
TWx38 Agrobacterium tumefaciens C58 wt
TWx39 Agrobacterium tumefaciens A136 rif Ti plasmid-cured derivative of strain C58
TWx40 Agrobacterium tumefaciens UBAPF I rif Plasmid free strain [7]
TWx41 Agrobacterium tumefaciens UBAPF II rif Plasmid free strain [7]
TWx42 Agrobacterium tumefaciens UBAPF I pTrc200 rif, strep, spec
TWx43 Agrobacterium tumefaciens UBAPF I pTrc200_dnaA rif, strep, spec IPTG inducible dnaA
TWx44 Agrobacterium tumefaciens UBAPF I pTrc200_dnaA_his rif, strep, spec IPTG inducible dnaA with his tag
TWx45 Agrobacterium tumefaciens UBAPF II pTrc200 rif, strep, spec
TWx46 Agrobacterium tumefaciens UBAPF II pTrc200_dnaA rif, strep, spec IPTG inducible dnaA
TWx47 Agrobacterium tumefaciens UBAPF II pTrc200_dnaA_his rif, strep, spec IPTG inducible dnaA with his tag
TWx48 MG1655 dnaC2 (R34) dnaC2 pMQ430 amp, tet For pMQ430 see TWx37
TWx49 MG1655 KS9921 (O19) delta seqA
TWx50 TWX49 (KS9921) ter::GATC-cluster cm Transfered by P1 transduction from TWx23
TWx51 TWX50 ter::GATC-cluster; pIO4 cm, amp seqA4 plasmid, IPTG inducible


  1. Hoskins JR, Singh SK, Maurizi MR, and Wickner S. . pmid:10922051. PubMed HubMed [Hoskins-2000]
  2. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. . pmid:15978072. PubMed HubMed [Bates2005MolMicro]
  3. Løbner-Olesen A, Marinus MG, and Hansen FG. . pmid:12682301. PubMed HubMed [Lobner-Olesen-2003]
  4. Parker B and Marinus MG. . pmid:2854098. PubMed HubMed [Parker-Marinus-88]
  5. Calmann MA and Marinus MG. . pmid:12897023. PubMed HubMed [Calmann-Marinus-03]
  6. Hynes MF, Simon R, and Pühler A. . pmid:4001194. PubMed HubMed [Puhler]
  7. Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. . pmid:6094968. PubMed HubMed [Hansen-1984]
All Medline abstracts: PubMed HubMed
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