User:Torsten Waldminghaus/Strains

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Current revision (08:17, 7 January 2013) (view source)
 
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Line 3: Line 3:
|-
|-
! TWx1
! TWx1
-
| DH5α  
+
| DH5α (Escherichia coli)
| supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96
| supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96
|no
|no
Line 9: Line 9:
|-
|-
! TWx2
! TWx2
-
|DH5α  
+
|DH5α (Escherichia coli) 
|pFH2102
|pFH2102
|amp
|amp
Line 15: Line 15:
|-
|-
! TWx3
! TWx3
-
|MG1655
+
|MG1655(Escherichia coli)
|?
|?
|no
|no
Line 21: Line 21:
|-
|-
! TWx4
! TWx4
-
|CM735
+
|CM735(Escherichia coli)
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 -
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 -
|no
|no
-
|<cite>Hansen-1989</cite>
+
|<cite>Hansen-1984</cite>
|-
|-
! TWx5
! TWx5
-
|DH5α
+
|DH5α(Escherichia coli)
|pGEM-pyrB
|pGEM-pyrB
|amp
|amp
Line 33: Line 33:
|-
|-
! TWx6
! TWx6
-
|DH5α
+
|DH5α(Escherichia coli)
|pFH2102-pyrB (=ppyrB)
|pFH2102-pyrB (=ppyrB)
|amp
|amp
Line 39: Line 39:
|-
|-
! TWx7
! TWx7
-
|SG2221T  
+
|SG2221T (Escherichia coli)
|pFH2102
|pFH2102
|amp
|amp
Line 45: Line 45:
|-
|-
! TWx8
! TWx8
-
|MG1655
+
|MG1655(Escherichia coli)
|pyrB::Tn5
|pyrB::Tn5
|kan
|kan
Line 65: Line 65:
|TW3 (MG1655)
|TW3 (MG1655)
|ppyrB
|ppyrB
-
|kan, amp
+
|amp
|
|
|-
|-
Line 71: Line 71:
|TW3 (MG1655)
|TW3 (MG1655)
|pFH2102 (empty vector)
|pFH2102 (empty vector)
-
|kan, amp
+
|amp
|
|
|-
|-
! TWx13
! TWx13
-
|CM735  
+
|CM735 (Escherichia coli)
|EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP
|EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP
|no
|no
Line 81: Line 81:
|-
|-
! TWx14
! TWx14
-
|DH5α
+
|DH5α(Escherichia coli)
|pET3a-Pfu
|pET3a-Pfu
|amp
|amp
Line 87: Line 87:
|-
|-
! TWx15
! TWx15
-
|DH5α
+
|DH5α(Escherichia coli)
|pTaq
|pTaq
|amp
|amp
Line 99: Line 99:
|-
|-
! TWx17
! TWx17
-
|MG1655
+
|MG1655(Escherichia coli)
|pGATC
|pGATC
|cm
|cm
Line 117: Line 117:
|-
|-
! TWx20
! TWx20
-
|MG1655
+
|MG1655(Escherichia coli)
|KS0003 (dam16) (box O33)
|KS0003 (dam16) (box O33)
|km
|km
Line 123: Line 123:
|-
|-
! TWx21
! TWx21
-
|DY330
+
|DY330(Escherichia coli)
|proAB::GATCcluster  
|proAB::GATCcluster  
|cm
|cm
Line 129: Line 129:
|-
|-
! TWx22
! TWx22
-
|DY330
+
|DY330(Escherichia coli)
|aroA::GATCcluster  
|aroA::GATCcluster  
|cm
|cm
Line 135: Line 135:
|-
|-
! TWx23
! TWx23
-
|DY330
+
|DY330(Escherichia coli)
|ter::GATCcluster  
|ter::GATCcluster  
|cm
|cm
Line 141: Line 141:
|-
|-
! TWx24
! TWx24
-
|DY330
+
|DY330(Escherichia coli)
|hisA::GATCcluster  
|hisA::GATCcluster  
|cm
|cm
Line 147: Line 147:
|-
|-
! TWx25
! TWx25
-
|DY330
+
|DY330(Escherichia coli)
|srlA::GATCcluster  
|srlA::GATCcluster  
|cm
|cm
Line 153: Line 153:
|-
|-
! TWx26
! TWx26
-
|MG1655
+
|MG1655(Escherichia coli)
|proAB::GATCcluster  
|proAB::GATCcluster  
|cm
|cm
Line 159: Line 159:
|-
|-
! TWx27
! TWx27
-
|MG1655
+
|MG1655(Escherichia coli)
|aroA::GATCcluster  
|aroA::GATCcluster  
|cm
|cm
Line 165: Line 165:
|-
|-
! TWx28
! TWx28
-
|MG1655
+
|MG1655(Escherichia coli)
|ter::GATCcluster (1)
|ter::GATCcluster (1)
|cm
|cm
Line 171: Line 171:
|-
|-
! TWx29
! TWx29
-
|MG1655
+
|MG1655(Escherichia coli)
|ter::GATCcluster (2)
|ter::GATCcluster (2)
|cm
|cm
Line 177: Line 177:
|-
|-
! TWx30
! TWx30
-
|MG1655
+
|MG1655(Escherichia coli)
|srlA::GATCcluster  
|srlA::GATCcluster  
|cm
|cm
Line 183: Line 183:
|-
|-
! TWx31
! TWx31
-
|MG1655
+
|MG1655(Escherichia coli)
|hisA:GATCcluster  
|hisA:GATCcluster  
|cm
|cm
Line 189: Line 189:
|-
|-
! TWx32
! TWx32
-
|CM735
+
|CM735(Escherichia coli)
|NK9386 Ptac::fliCst
|NK9386 Ptac::fliCst
|no
|no
Line 195: Line 195:
|-
|-
! TWx33
! TWx33
-
|MG1655
+
|MG1655(Escherichia coli)
|ter::GATCcluster (ter1)
|ter::GATCcluster (ter1)
|cm
|cm
Line 201: Line 201:
|-
|-
! TWx34
! TWx34
-
|MG1655
+
|MG1655(Escherichia coli)
|ter::GATCcluster (ter2)
|ter::GATCcluster (ter2)
|cm
|cm
Line 207: Line 207:
|-
|-
! TWx35
! TWx35
-
|MG1655
+
|MG1655(Escherichia coli)
|ALO1739 (arok17::cat/pMS2)
|ALO1739 (arok17::cat/pMS2)
|cm, km
|cm, km
Line 225: Line 225:
|-
|-
! TWx38
! TWx38
 +
|Agrobacterium tumefaciens C58
|
|
|
|
-
|
+
|wt
-
|
+
|-
|-
! TWx39
! TWx39
 +
|Agrobacterium tumefaciens A136
 +
|rif
|
|
-
|
+
|Ti plasmid-cured derivative of strain C58
-
|
+
-
|
+
|-
|-
! TWx40
! TWx40
 +
|Agrobacterium tumefaciens UBAPF I
 +
|rif
|
|
-
|
+
|Plasmid free strain <cite>Puhler</cite>
-
|
+
-
|
+
|-
|-
! TWx41
! TWx41
 +
|Agrobacterium tumefaciens UBAPF II
 +
|rif
|
|
-
|
+
|Plasmid free strain <cite>Puhler</cite>
-
|
+
-
|
+
|-
|-
! TWx42
! TWx42
-
|
+
|Agrobacterium tumefaciens UBAPF I
-
|
+
|pTrc200
-
|
+
|rif, strep, spec
|
|
|-
|-
! TWx43
! TWx43
-
|
+
|Agrobacterium tumefaciens UBAPF I
-
|
+
|pTrc200_dnaA
-
|
+
|rif, strep, spec
-
|
+
|IPTG inducible dnaA
|-
|-
! TWx44
! TWx44
-
|
+
|Agrobacterium tumefaciens UBAPF I
-
|
+
|pTrc200_dnaA_his
-
|
+
|rif, strep, spec
-
|
+
|IPTG inducible dnaA with his tag
|-
|-
! TWx45
! TWx45
-
|
+
|Agrobacterium tumefaciens UBAPF II
-
|
+
|pTrc200
-
|
+
|rif, strep, spec
|
|
|-
|-
! TWx46
! TWx46
-
|
+
|Agrobacterium tumefaciens UBAPF II
-
|
+
|pTrc200_dnaA
-
|
+
|rif, strep, spec
-
|
+
|IPTG inducible dnaA
|-
|-
! TWx47
! TWx47
-
|
+
|Agrobacterium tumefaciens UBAPF II
-
|
+
|pTrc200_dnaA_his
-
|
+
|rif, strep, spec
-
|
+
|IPTG inducible dnaA with his tag
|-
|-
! TWx48
! TWx48
Line 291: Line 291:
|-
|-
! TWx49
! TWx49
-
|
+
|MG1655(Escherichia coli)
-
|
+
|KS9921 (O19) delta seqA
|
|
|
|
|-
|-
! TWx50
! TWx50
-
|
+
|TWX49 (KS9921)
-
|
+
|ter::GATC-cluster
-
|
+
|cm
-
|
+
|Transfered by P1 transduction from TWx23
 +
|-
 +
! TWx51
 +
|TWX50
 +
|ter::GATC-cluster; pIO4
 +
|cm, amp
 +
|seqA4 plasmid, IPTG inducible
 +
|-
 +
! TWx52
 +
|AB1157(Escherichia coli)
 +
|Ab75; MukB L1407Q GFP
 +
|cm
 +
|from David Sherratt
 +
|-
 +
! TWx53
 +
|MG1655 delta dam
 +
|proAB::GATC-cluster-1
 +
|cm, km
 +
|from Martina, not checked if correct
 +
|-
 +
! TWx54
 +
|MG1655 delta dam
 +
|proAB::GATC-cluster-2
 +
|cm, km
 +
|as TWX53 from other transduction
 +
|-
 +
! TWx55
 +
|MG1655 dnaC2
 +
|proAB::GATC-cluster
 +
|cm, tet
 +
|from Martina, not checked if correct
 +
|-
 +
! TWx56
 +
|MG1655 dnaC2
 +
|terC::GATC-cluster
 +
|cm, tet
 +
|from Martina, not checked if correct
 +
|-
 +
! TWx57
 +
|MG1655 dnaC2
 +
|srlA::GATC-cluster
 +
|cm, tet
 +
|from Martina, not checked if correct
 +
|-
 +
! TWx58
 +
|MG1655 dnaC2
 +
|hisA::GATC-cluster
 +
|cm, tet
 +
|from Martina, not checked if correct
 +
|-
 +
! TW200
 +
|DH5α
 +
|pWH1866 (pUC18 derivative, InsTetG−1 flanked by PvuII sites)
 +
|amp, kan
 +
|<cite>Koestner</cite>
 +
|-
 +
! TW201
 +
|DH5α
 +
|pWH1867 (pCB302b derivative, InsTetG−1 upstream of a promoterless lacZ)
 +
|amp, kan
 +
|<cite>Koestner</cite>
 +
|-
 +
! TW202
 +
|DH5α
 +
|pWH1891 (pTYB2 derivative, Tn5 transposase E54K, M56A, L372P; C-terminal intein-chitin binding domain fusion)
 +
|amp
 +
|<cite>Koestner</cite>
|-
|-
|}
|}
Line 312: Line 378:
#Parker-Marinus-88 pmid=2854098  
#Parker-Marinus-88 pmid=2854098  
#Calmann-Marinus-03 pmid=12897023
#Calmann-Marinus-03 pmid=12897023
 +
#Puhler pmid=4001194
 +
#Koestner pmid=16820464
</biblio>
</biblio>

Current revision

Strain Parental strain Characteristics AB resistence Reference
TWx1 DH5α (Escherichia coli) supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 no
TWx2 DH5α (Escherichia coli) pFH2102 amp
TWx3 MG1655(Escherichia coli) ? no
TWx4 CM735(Escherichia coli) metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - no [1]
TWx5 DH5α(Escherichia coli) pGEM-pyrB amp
TWx6 DH5α(Escherichia coli) pFH2102-pyrB (=ppyrB) amp
TWx7 SG2221T (Escherichia coli) pFH2102 amp [2]
TWx8 MG1655(Escherichia coli) pyrB::Tn5 kan Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain)
TWx9 TW8 pyrB::Tn5 ppyrB kan, amp
TWx10 TW8 pyrB::Tn5 pFH2102 (empty vector) kan, amp
TWx11 TW3 (MG1655) ppyrB amp
TWx12 TW3 (MG1655) pFH2102 (empty vector) amp
TWx13 CM735 (Escherichia coli) EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP no
TWx14 DH5α(Escherichia coli) pET3a-Pfu amp strain to overproduce and purify Pfu DNA polymerase
TWx15 DH5α(Escherichia coli) pTaq amp strain to overproduce and purify Taq DNA polymerase
TWx16 KS0003 MG1655 dam16 pGATC km, cm
TWx17 MG1655(Escherichia coli) pGATC cm
TWx18 ALO1826 (box R41) amp
TWx19 ALO1829 (box R43) amp, cm
TWx20 MG1655(Escherichia coli) KS0003 (dam16) (box O33) km
TWx21 DY330(Escherichia coli) proAB::GATCcluster cm
TWx22 DY330(Escherichia coli) aroA::GATCcluster cm
TWx23 DY330(Escherichia coli) ter::GATCcluster cm
TWx24 DY330(Escherichia coli) hisA::GATCcluster cm
TWx25 DY330(Escherichia coli) srlA::GATCcluster cm
TWx26 MG1655(Escherichia coli) proAB::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta
TWx27 MG1655(Escherichia coli) aroA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta
TWx28 MG1655(Escherichia coli) ter::GATCcluster (1) cm Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx29 MG1655(Escherichia coli) ter::GATCcluster (2) cm Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx30 MG1655(Escherichia coli) srlA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta
TWx31 MG1655(Escherichia coli) hisA:GATCcluster cm Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta
TWx32 CM735(Escherichia coli) NK9386 Ptac::fliCst no strain with IPTG inducible stick flagella for baby cell column [3]
TWx33 MG1655(Escherichia coli) ter::GATCcluster (ter1) cm Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx34 MG1655(Escherichia coli) ter::GATCcluster (ter2) cm Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx35 MG1655(Escherichia coli) ALO1739 (arok17::cat/pMS2) cm, km strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4]
TWx36 GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51) km The prototype dam-16::Kan deletion strain [5] http://users.umassmed.edu/martin.marinus/dstrains.html
TWx37 GM3819 pMQ430/GM3819 km, amp pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6] http://users.umassmed.edu/martin.marinus/plasmid.html
TWx38 Agrobacterium tumefaciens C58 wt
TWx39 Agrobacterium tumefaciens A136 rif Ti plasmid-cured derivative of strain C58
TWx40 Agrobacterium tumefaciens UBAPF I rif Plasmid free strain [7]
TWx41 Agrobacterium tumefaciens UBAPF II rif Plasmid free strain [7]
TWx42 Agrobacterium tumefaciens UBAPF I pTrc200 rif, strep, spec
TWx43 Agrobacterium tumefaciens UBAPF I pTrc200_dnaA rif, strep, spec IPTG inducible dnaA
TWx44 Agrobacterium tumefaciens UBAPF I pTrc200_dnaA_his rif, strep, spec IPTG inducible dnaA with his tag
TWx45 Agrobacterium tumefaciens UBAPF II pTrc200 rif, strep, spec
TWx46 Agrobacterium tumefaciens UBAPF II pTrc200_dnaA rif, strep, spec IPTG inducible dnaA
TWx47 Agrobacterium tumefaciens UBAPF II pTrc200_dnaA_his rif, strep, spec IPTG inducible dnaA with his tag
TWx48 MG1655 dnaC2 (R34) dnaC2 pMQ430 amp, tet For pMQ430 see TWx37
TWx49 MG1655(Escherichia coli) KS9921 (O19) delta seqA
TWx50 TWX49 (KS9921) ter::GATC-cluster cm Transfered by P1 transduction from TWx23
TWx51 TWX50 ter::GATC-cluster; pIO4 cm, amp seqA4 plasmid, IPTG inducible
TWx52 AB1157(Escherichia coli) Ab75; MukB L1407Q GFP cm from David Sherratt
TWx53 MG1655 delta dam proAB::GATC-cluster-1 cm, km from Martina, not checked if correct
TWx54 MG1655 delta dam proAB::GATC-cluster-2 cm, km as TWX53 from other transduction
TWx55 MG1655 dnaC2 proAB::GATC-cluster cm, tet from Martina, not checked if correct
TWx56 MG1655 dnaC2 terC::GATC-cluster cm, tet from Martina, not checked if correct
TWx57 MG1655 dnaC2 srlA::GATC-cluster cm, tet from Martina, not checked if correct
TWx58 MG1655 dnaC2 hisA::GATC-cluster cm, tet from Martina, not checked if correct
TW200 DH5α pWH1866 (pUC18 derivative, InsTetG−1 flanked by PvuII sites) amp, kan [8]
TW201 DH5α pWH1867 (pCB302b derivative, InsTetG−1 upstream of a promoterless lacZ) amp, kan [8]
TW202 DH5α pWH1891 (pTYB2 derivative, Tn5 transposase E54K, M56A, L372P; C-terminal intein-chitin binding domain fusion) amp [8]


  1. Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. . pmid:6094968. PubMed HubMed [Hansen-1984]
  2. Hoskins JR, Singh SK, Maurizi MR, and Wickner S. . pmid:10922051. PubMed HubMed [Hoskins-2000]
  3. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. . pmid:15978072. PubMed HubMed [Bates2005MolMicro]
  4. Løbner-Olesen A, Marinus MG, and Hansen FG. . pmid:12682301. PubMed HubMed [Lobner-Olesen-2003]
  5. Parker B and Marinus MG. . pmid:2854098. PubMed HubMed [Parker-Marinus-88]
  6. Calmann MA and Marinus MG. . pmid:12897023. PubMed HubMed [Calmann-Marinus-03]
  7. Hynes MF, Simon R, and Pühler A. . pmid:4001194. PubMed HubMed [Puhler]
  8. Köstner M, Schmidt B, Bertram R, and Hillen W. . pmid:16820464. PubMed HubMed [Koestner]
All Medline abstracts: PubMed HubMed
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