User:Torsten Waldminghaus/Strains: Difference between revisions
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(New page: {| border="3" ! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference |- ! ''TW1'' | DH5α | ? |no | |- ! ''TW2'' |DH5α |pFH2102 |amp | |- ! ''TW3'' |MG1655 |? |no ...) |
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! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference | ! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference | ||
|- | |- | ||
! | ! TW1 | ||
| DH5α | | DH5α | ||
| | | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | ||
|no | |no | ||
| | | | ||
|- | |- | ||
! | ! TW2 | ||
|DH5α | |DH5α | ||
|pFH2102 | |pFH2102 | ||
| Line 14: | Line 14: | ||
| | | | ||
|- | |- | ||
! | ! TW3 | ||
|MG1655 | |MG1655 | ||
|? | |? | ||
| Line 20: | Line 20: | ||
| | | | ||
|- | |- | ||
! | ! TW4 | ||
|CM735 | |CM735 | ||
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | |metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | ||
|no | |no | ||
| | |<cite>Hansen-1989</cite> | ||
|- | |- | ||
! | ! TW5 | ||
|DH5α | |DH5α | ||
|pGEM-pyrB | |pGEM-pyrB | ||
| Line 32: | Line 32: | ||
| | | | ||
|- | |- | ||
! | ! TW6 | ||
|DH5α | |DH5α | ||
|pFH2102-pyrB (=ppyrB) | |pFH2102-pyrB (=ppyrB) | ||
| Line 38: | Line 38: | ||
| | | | ||
|- | |- | ||
! | ! TW7 | ||
|SG2221T | |SG2221T | ||
|pFH2102 | |pFH2102 | ||
|amp | |amp | ||
|<cite>Hoskins-2000</cite> | |<cite>Hoskins-2000</cite> | ||
|- | |||
! TW8 | |||
|MG1655 | |||
|pyrB::Tn5 | |||
|kan | |||
|Transfered to MG1655 by P1 transduction from ME8359 ([http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp National BioResource Project E.coli Strain]) | |||
|- | |||
! TW9 | |||
|TW8 | |||
|pyrB::Tn5 ppyrB | |||
|kan, amp | |||
| | |||
|- | |||
! TW10 | |||
|TW8 | |||
|pyrB::Tn5 pFH2102 (empty vector) | |||
|kan, amp | |||
| | |||
|- | |||
! TW11 | |||
|TW3 (MG1655) | |||
|ppyrB | |||
|kan, amp | |||
| | |||
|- | |||
! TW12 | |||
|TW3 (MG1655) | |||
|pFH2102 (empty vector) | |||
|kan, amp | |||
| | |||
|- | |- | ||
|} | |} | ||
| Line 49: | Line 79: | ||
<biblio> | <biblio> | ||
#Hoskins-2000 pmid=10922051 | #Hoskins-2000 pmid=10922051 | ||
#Hansen-1984 pmid=6094968 | |||
</biblio> | </biblio> | ||
Revision as of 06:34, 28 April 2008
| Strain | Parental strain | Characteristics | AB resistence | Reference |
|---|---|---|---|---|
| TW1 | DH5α | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | no | |
| TW2 | DH5α | pFH2102 | amp | |
| TW3 | MG1655 | ? | no | |
| TW4 | CM735 | metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | no | [1] |
| TW5 | DH5α | pGEM-pyrB | amp | |
| TW6 | DH5α | pFH2102-pyrB (=ppyrB) | amp | |
| TW7 | SG2221T | pFH2102 | amp | [2] |
| TW8 | MG1655 | pyrB::Tn5 | kan | Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain) |
| TW9 | TW8 | pyrB::Tn5 ppyrB | kan, amp | |
| TW10 | TW8 | pyrB::Tn5 pFH2102 (empty vector) | kan, amp | |
| TW11 | TW3 (MG1655) | ppyrB | kan, amp | |
| TW12 | TW3 (MG1655) | pFH2102 (empty vector) | kan, amp |
- Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. DOI:10.1073/pnas.97.16.8892 |
- Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. DOI:10.1007/BF00436184 |
