User:Torsten Waldminghaus/Strains: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 170: Line 170:
|Transfered to MG1655 by P1 transduction from TWx23
|Transfered to MG1655 by P1 transduction from TWx23
|-
|-
! TWx26
! TWx29
|MG1655
|MG1655
|ter::GATCcluster (2)
|ter::GATCcluster (2)
Line 176: Line 176:
|Transfered to MG1655 by P1 transduction from TWx23
|Transfered to MG1655 by P1 transduction from TWx23
|-
|-
! TWx26
! TWx30
|MG1655
|MG1655
|srlA::GATCcluster  
|srlA::GATCcluster  
Line 182: Line 182:
|Transfered to MG1655 by P1 transduction from TWx25
|Transfered to MG1655 by P1 transduction from TWx25
|-
|-
! TWx27
! TWx31
|
|MG1655
|
|hisA:GATCcluster
|
|cm
|
|Transfered to MG1655 by P1 transduction from TWx24
|-
|-
! TWx28
! TWx32
|
|CM735
|
|NK9386
|
|no
|
|strain with IPTG inducible stick flagella for baby cell column <cite>Bates2005MolMicro</cite>
|-
|-
! TWx29
! TWx33
|
|MG1655
|
|ter::GATCcluster (ter1)
|
|cm
|
|Transfered to MG1655 by P1 transduction from TWx23
|-
|-
! TWx30
! TWx34
|
|MG1655
|
|ter::GATCcluster (ter2)
|
|cm
|
|Transfered to MG1655 by P1 transduction from TWx23
|-
|-
! TWx31
! TWx35
|
|MG1655
|
|ALO1739 (arok17::cat/pMS2)
|
|cm, km
|
|strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in <cite>Lobner-Olesen-2003</cite>
|-
|-
! TWx32
! TWx36
|
|
|
|
|
|GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51)
|km
|The prototype dam-16::Kan deletion strain <cite>Parker-Marinus-88</cite>.
|-
|-
! TWx33
! TWx37
|
|GM3819
|
|pMQ430/GM3819
|
|km, cm
|
|pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence <cite>Calmann-Marinus-03</cite>
|-
|-
|}
|}
Line 230: Line 230:
#Hoskins-2000 pmid=10922051
#Hoskins-2000 pmid=10922051
#Hansen-1984 pmid=6094968
#Hansen-1984 pmid=6094968
#Bates2005MolMicro pmid=15978072
#Lobner-Olesen-2003 pmid=12682301
#Parker-Marinus-88 pmid=2854098
#Calmann-Marinus-03 pmid=12897023
</biblio>
</biblio>

Revision as of 03:41, 27 January 2010

Strain Parental strain Characteristics AB resistence Reference
TWx1 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 no
TWx2 DH5α pFH2102 amp
TWx3 MG1655 ? no
TWx4 CM735 metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - no [1]
TWx5 DH5α pGEM-pyrB amp
TWx6 DH5α pFH2102-pyrB (=ppyrB) amp
TWx7 SG2221T pFH2102 amp [2]
TWx8 MG1655 pyrB::Tn5 kan Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain)
TWx9 TW8 pyrB::Tn5 ppyrB kan, amp
TWx10 TW8 pyrB::Tn5 pFH2102 (empty vector) kan, amp
TWx11 TW3 (MG1655) ppyrB kan, amp
TWx12 TW3 (MG1655) pFH2102 (empty vector) kan, amp
TWx13
TWx14
TWx15
TWx16 KS0006 MG1655 Δdam no no
TWx17 MG1655 pGATC cm
TWx18 ALO1826 (box R41) amp
TWx19 ALO1829 (box R43) amp, cm
TWx20
TWx21 DY330 proAB::GATCcluster cm
TWx22 DY330 aroA::GATCcluster cm
TWx23 DY330 ter::GATCcluster cm
TWx24 DY330 hisA::GATCcluster cm
TWx25 DY330 srlA::GATCcluster cm
TWx26 MG1655 proAB::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta
TWx27 MG1655 aroA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx22
TWx28 MG1655 ter::GATCcluster (1) cm Transfered to MG1655 by P1 transduction from TWx23
TWx29 MG1655 ter::GATCcluster (2) cm Transfered to MG1655 by P1 transduction from TWx23
TWx30 MG1655 srlA::GATCcluster cm Transfered to MG1655 by P1 transduction from TWx25
TWx31 MG1655 hisA:GATCcluster cm Transfered to MG1655 by P1 transduction from TWx24
TWx32 CM735 NK9386 no strain with IPTG inducible stick flagella for baby cell column [3]
TWx33 MG1655 ter::GATCcluster (ter1) cm Transfered to MG1655 by P1 transduction from TWx23
TWx34 MG1655 ter::GATCcluster (ter2) cm Transfered to MG1655 by P1 transduction from TWx23
TWx35 MG1655 ALO1739 (arok17::cat/pMS2) cm, km strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4]
TWx36 GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51) km The prototype dam-16::Kan deletion strain [5].
TWx37 GM3819 pMQ430/GM3819 km, cm pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6]


  1. Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. DOI:10.1073/pnas.97.16.8892 | PubMed ID:10922051 | HubMed [Hoskins-2000]
  2. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle. Mol Microbiol. 2005 Jul;57(2):380-91. DOI:10.1111/j.1365-2958.2005.04693.x | PubMed ID:15978072 | HubMed [Bates2005MolMicro]
  3. Løbner-Olesen A, Marinus MG, and Hansen FG. Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4672-7. DOI:10.1073/pnas.0538053100 | PubMed ID:12682301 | HubMed [Lobner-Olesen-2003]
  4. Parker B and Marinus MG. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Gene. 1988 Dec 20;73(2):531-5. DOI:10.1016/0378-1119(88)90517-3 | PubMed ID:2854098 | HubMed [Parker-Marinus-88]
  5. Calmann MA and Marinus MG. Regulated expression of the Escherichia coli dam gene. J Bacteriol. 2003 Aug;185(16):5012-4. DOI:10.1128/JB.185.16.5012-5014.2003 | PubMed ID:12897023 | HubMed [Calmann-Marinus-03]
  6. Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. DOI:10.1007/BF00436184 | PubMed ID:6094968 | HubMed [Hansen-1984]

All Medline abstracts: PubMed | HubMed

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Torsten_Waldminghaus/Strains&oldid=385296"