User:Torsten Waldminghaus/Strains: Difference between revisions
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|cm, tet | |cm, tet | ||
|from Martina, not checked if correct | |from Martina, not checked if correct | ||
|- | |||
! TW200 | |||
|DH5α | |||
|pWH1866 (pUC18 derivative, InsTetG−1 flanked by PvuII sites) | |||
|amp, kan | |||
|<cite>Koestner</cite> | |||
|- | |||
! TW201 | |||
|DH5α | |||
|pWH1867 (pCB302b derivative, InsTetG−1 upstream of a promoterless lacZ) | |||
|amp, kan | |||
|<cite>Koestner</cite> | |||
|- | |||
! TW202 | |||
|DH5α | |||
|pWH1891 (pTYB2 derivative, Tn5 transposase E54K, M56A, L372P; C-terminal intein-chitin binding domain fusion) | |||
|amp | |||
|<cite>Koestner</cite> | |||
|- | |- | ||
|} | |} | ||
| Line 361: | Line 379: | ||
#Calmann-Marinus-03 pmid=12897023 | #Calmann-Marinus-03 pmid=12897023 | ||
#Puhler pmid=4001194 | #Puhler pmid=4001194 | ||
#Koestner pmid=16820464 | |||
</biblio> | </biblio> | ||
Revision as of 01:35, 5 July 2012
| Strain | Parental strain | Characteristics | AB resistence | Reference |
|---|---|---|---|---|
| TWx1 | DH5α (Escherichia coli) | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | no | |
| TWx2 | DH5α (Escherichia coli) | pFH2102 | amp | |
| TWx3 | MG1655(Escherichia coli) | ? | no | |
| TWx4 | CM735(Escherichia coli) | metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | no | [1] |
| TWx5 | DH5α(Escherichia coli) | pGEM-pyrB | amp | |
| TWx6 | DH5α(Escherichia coli) | pFH2102-pyrB (=ppyrB) | amp | |
| TWx7 | SG2221T (Escherichia coli) | pFH2102 | amp | [2] |
| TWx8 | MG1655(Escherichia coli) | pyrB::Tn5 | kan | Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain) |
| TWx9 | TW8 | pyrB::Tn5 ppyrB | kan, amp | |
| TWx10 | TW8 | pyrB::Tn5 pFH2102 (empty vector) | kan, amp | |
| TWx11 | TW3 (MG1655) | ppyrB | amp | |
| TWx12 | TW3 (MG1655) | pFH2102 (empty vector) | amp | |
| TWx13 | CM735 (Escherichia coli) | EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP | no | |
| TWx14 | DH5α(Escherichia coli) | pET3a-Pfu | amp | strain to overproduce and purify Pfu DNA polymerase |
| TWx15 | DH5α(Escherichia coli) | pTaq | amp | strain to overproduce and purify Taq DNA polymerase |
| TWx16 | KS0003 | MG1655 dam16 pGATC | km, cm | |
| TWx17 | MG1655(Escherichia coli) | pGATC | cm | |
| TWx18 | ALO1826 (box R41) | amp | ||
| TWx19 | ALO1829 (box R43) | amp, cm | ||
| TWx20 | MG1655(Escherichia coli) | KS0003 (dam16) (box O33) | km | |
| TWx21 | DY330(Escherichia coli) | proAB::GATCcluster | cm | |
| TWx22 | DY330(Escherichia coli) | aroA::GATCcluster | cm | |
| TWx23 | DY330(Escherichia coli) | ter::GATCcluster | cm | |
| TWx24 | DY330(Escherichia coli) | hisA::GATCcluster | cm | |
| TWx25 | DY330(Escherichia coli) | srlA::GATCcluster | cm | |
| TWx26 | MG1655(Escherichia coli) | proAB::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta |
| TWx27 | MG1655(Escherichia coli) | aroA::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta |
| TWx28 | MG1655(Escherichia coli) | ter::GATCcluster (1) | cm | Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx29 | MG1655(Escherichia coli) | ter::GATCcluster (2) | cm | Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx30 | MG1655(Escherichia coli) | srlA::GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta |
| TWx31 | MG1655(Escherichia coli) | hisA:GATCcluster | cm | Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta |
| TWx32 | CM735(Escherichia coli) | NK9386 Ptac::fliCst | no | strain with IPTG inducible stick flagella for baby cell column [3] |
| TWx33 | MG1655(Escherichia coli) | ter::GATCcluster (ter1) | cm | Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx34 | MG1655(Escherichia coli) | ter::GATCcluster (ter2) | cm | Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion |
| TWx35 | MG1655(Escherichia coli) | ALO1739 (arok17::cat/pMS2) | cm, km | strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4] |
| TWx36 | GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51) | km | The prototype dam-16::Kan deletion strain [5] http://users.umassmed.edu/martin.marinus/dstrains.html | |
| TWx37 | GM3819 | pMQ430/GM3819 | km, amp | pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6] http://users.umassmed.edu/martin.marinus/plasmid.html |
| TWx38 | Agrobacterium tumefaciens C58 | wt | ||
| TWx39 | Agrobacterium tumefaciens A136 | rif | Ti plasmid-cured derivative of strain C58 | |
| TWx40 | Agrobacterium tumefaciens UBAPF I | rif | Plasmid free strain [7] | |
| TWx41 | Agrobacterium tumefaciens UBAPF II | rif | Plasmid free strain [7] | |
| TWx42 | Agrobacterium tumefaciens UBAPF I | pTrc200 | rif, strep, spec | |
| TWx43 | Agrobacterium tumefaciens UBAPF I | pTrc200_dnaA | rif, strep, spec | IPTG inducible dnaA |
| TWx44 | Agrobacterium tumefaciens UBAPF I | pTrc200_dnaA_his | rif, strep, spec | IPTG inducible dnaA with his tag |
| TWx45 | Agrobacterium tumefaciens UBAPF II | pTrc200 | rif, strep, spec | |
| TWx46 | Agrobacterium tumefaciens UBAPF II | pTrc200_dnaA | rif, strep, spec | IPTG inducible dnaA |
| TWx47 | Agrobacterium tumefaciens UBAPF II | pTrc200_dnaA_his | rif, strep, spec | IPTG inducible dnaA with his tag |
| TWx48 | MG1655 dnaC2 (R34) | dnaC2 pMQ430 | amp, tet | For pMQ430 see TWx37 |
| TWx49 | MG1655(Escherichia coli) | KS9921 (O19) delta seqA | ||
| TWx50 | TWX49 (KS9921) | ter::GATC-cluster | cm | Transfered by P1 transduction from TWx23 |
| TWx51 | TWX50 | ter::GATC-cluster; pIO4 | cm, amp | seqA4 plasmid, IPTG inducible |
| TWx52 | AB1157(Escherichia coli) | Ab75; MukB L1407Q GFP | cm | from David Sherratt |
| TWx53 | MG1655 delta dam | proAB::GATC-cluster-1 | cm, km | from Martina, not checked if correct |
| TWx54 | MG1655 delta dam | proAB::GATC-cluster-2 | cm, km | as TWX53 from other transduction |
| TWx55 | MG1655 dnaC2 | proAB::GATC-cluster | cm, tet | from Martina, not checked if correct |
| TWx56 | MG1655 dnaC2 | terC::GATC-cluster | cm, tet | from Martina, not checked if correct |
| TWx57 | MG1655 dnaC2 | srlA::GATC-cluster | cm, tet | from Martina, not checked if correct |
| TWx58 | MG1655 dnaC2 | hisA::GATC-cluster | cm, tet | from Martina, not checked if correct |
| TW200 | DH5α | pWH1866 (pUC18 derivative, InsTetG−1 flanked by PvuII sites) | amp, kan | [8] |
| TW201 | DH5α | pWH1867 (pCB302b derivative, InsTetG−1 upstream of a promoterless lacZ) | amp, kan | [8] |
| TW202 | DH5α | pWH1891 (pTYB2 derivative, Tn5 transposase E54K, M56A, L372P; C-terminal intein-chitin binding domain fusion) | amp | [8] |
- Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. DOI:10.1073/pnas.97.16.8892 |
- Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle. Mol Microbiol. 2005 Jul;57(2):380-91. DOI:10.1111/j.1365-2958.2005.04693.x |
- Løbner-Olesen A, Marinus MG, and Hansen FG. Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4672-7. DOI:10.1073/pnas.0538053100 |
- Parker B and Marinus MG. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Gene. 1988 Dec 20;73(2):531-5. DOI:10.1016/0378-1119(88)90517-3 |
- Calmann MA and Marinus MG. Regulated expression of the Escherichia coli dam gene. J Bacteriol. 2003 Aug;185(16):5012-4. DOI:10.1128/JB.185.16.5012-5014.2003 |
- Hynes MF, Simon R, and Pühler A. The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58. Plasmid. 1985 Mar;13(2):99-105. DOI:10.1016/0147-619x(85)90062-9 |
- Köstner M, Schmidt B, Bertram R, and Hillen W. Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase. Appl Environ Microbiol. 2006 Jul;72(7):4717-25. DOI:10.1128/AEM.00492-06 |
- Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. DOI:10.1007/BF00436184 |
