User:Torsten Waldminghaus/flow cytometry notes

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Revision as of 07:19, 2 April 2008 by Torsten Waldminghaus (Talk | contribs)
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Purpose

  • TBS stands for tris-buffered saline and is used in many protocols for washing


Recipe

  • 1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab
1xTBS
for 500 ml
10 ml 1M Tris-HCl pH 7.5
13 ml 5M NaCl
477 ml ddH2O
  • Prepare and sterile filter. Store at 4°C.
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