User:Torsten Waldminghaus/protein gel western
From OpenWetWare
Jump to navigationJump to search
Recipe for buffers
20 ml TE buffer |
5 ml Stacking gel buffer |
3.5 ml 10% SDS |
1.5 ml glycerol |
4 ml ddH2O |
- Store at rt.
285 μL 2.5M DTT |
205 μL 10%SDS |
10 μL 10% Bromphenolblue |
- Store at -20°C
- Should be 15% of endvolume (for example 42.5 μL sample + 7.5 μL Loading buffer