User:Torsten Waldminghaus/qPCR-Primers

Name	Sequence	Characteristics	Probe set number
uvrDfw	AGTTCCCGCAGGTGTTTATC	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])	1
uvrDrv	GTCAGCGTCAGTTTCTGCAT	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])	1
uvrDprobe	AGACGCCCGCCTTCATCCAG	HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region on E. coli chromosome with no GATC-sites (Region B from [1]	1
yahEFfw	CCATCGAGACGATCAAAGAA	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
yahEFrv	CAGCATCTGGCTTTGTTGTT	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
yahEFprobe	AACTCGCGTCCTTCGGCAGC	HPLC5'Fam - 3'Tamra qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
cluster-fw	CTGACTGATGAGATCCAACGA	qPCR for GATC-cluster	14
cluster-rv	CTGGTGCTACGCCTGAATAA	qPCR for GATC-cluster	14
cluster-p	AAATTCGACCCGGCTGTCGC	qPCR for GATC-cluster	14
761139p	CCAGGAAGCCCACGGATTCG		6
761139fw	GAGATCCTGCCGATGATGTA		6
761139rv	TTCCAGCAACTCTTTGATCG		6
797735fw	CGTCCTGGCGTATCGTATC		4
797735rv	GCATTGTAAGAACCTACAAAGACAA		4
797735p	TTTGCCGCAGAGTCTGCGCT		4
1504230fw	CGCCTTCAGTTTATGATCCA		13
1504230rv	TCGAGAAGTGTTCAAAGCAGA		13
1504230p	TGATCACCATCGCCTGCTGTTG		13
1514191fw	ATACTGTTTGGCAGAGGCAA		12
1514191rv	GGTATGGCTGATGATGTGCT		12
1514191p	TGACCGGTCAGCGGATCACC		12
2318305fw	ATAATCACCTACGCGCCTTC		5
2318305rv	ACCTGCCTGCCTGAATAAAC		5
2318305p	TGCCGCAGATCACCCTGGTC		5
2351285fw	ACACATTGCCGAATATGCC		16
2351285rv	GTTAATGCGGTGATCCATGA		16
2351285p	CTGCGCATTCGCATGTTCCC		16
2923803fw	GTCAGCCACCTGCTGAAAT		15
2923803rv	ATACTGAATTTGGAGCGAACC		15
2923803p	TGCCTGATCACCCATCAACCAGA		15
2952960fw	CTACTGTTTCGCGGATCTCA		8
2952960rv	CAACTGCTTGCAGAACCATT		8
2952960p	CTGGTGATCACCCGCGCATT		8
3923874fw	GCCCTGTGGATAACAAGGAT		18
3923874rv	CCTCATTCTGATCCCAGCTT		18
3923874p	CGGTCCAGGATCACCGATCATTC		18
3921366fw	GAGAATATGGCGTACCAGCA		7
3921366rv	AAGACGCAGGTATTTCGCTT		7
3921366p	CAACCTGACTTCGGTCCGCG		7
4301774fw	CTGAATAACTCGCCTCGTGA		10
4301774rv	ATTCCCGTCTTCATGGTTTC		10
4301774p	TAAGCGCCGATCACCGGGAT		10
4321507fw	AGCCAGAGGTGGAGTTAGGA		9
4321507rv	ACAACCTGAACGATCTGCTG		9
4321507p	TCTCACCTTTGGCAATGGCGA		9
ter-fw	TCCTCGCTGTTTGTCATCTT		17
ter-rv	GGTCTTGCTCGAATCCCTT		17
ter-p	CATCAGCACCCACGCAGCAA		17

1. Yamazoe M, Adachi S, Kanaya S, Ohsumi K, and Hiraga S. Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli. Mol Microbiol. 2005 Jan;55(1):289-98. DOI:10.1111/j.1365-2958.2004.04389.x |