User:Torsten Waldminghaus/qPCR-Primers

Name	Sequence	Characteristics	Probe set number
uvrDfw	AGTTCCCGCAGGTGTTTATC	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])	1
uvrDrv	GTCAGCGTCAGTTTCTGCAT	qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])	1
uvrDprobe	AGACGCCCGCCTTCATCCAG	HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region on E. coli chromosome with no GATC-sites (Region B from [1]	1
yahEFfw	CCATCGAGACGATCAAAGAA	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
yahEFrv	CAGCATCTGGCTTTGTTGTT	qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
yahEFprobe	AACTCGCGTCCTTCGGCAGC	HPLC5'Fam - 3'Tamra qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])	2
cluster-fw	CTGACTGATGAGATCCAACGA	qPCR for GATC-cluster	14
cluster-rv	CTGGTGCTACGCCTGAATAA	qPCR for GATC-cluster	14
cluster-p	AAATTCGACCCGGCTGTCGC	qPCR for GATC-cluster	14
761139p	CCAGGAAGCCCACGGATTCG
761139fw	GAGATCCTGCCGATGATGTA
761139rv	TTCCAGCAACTCTTTGATCG
797735fw	CGTCCTGGCGTATCGTATC
797735rv	GCATTGTAAGAACCTACAAAGACAA
797735p	TTTGCCGCAGAGTCTGCGCT
1504230fw	CGCCTTCAGTTTATGATCCA
1504230rv	TCGAGAAGTGTTCAAAGCAGA
1504230p	TGATCACCATCGCCTGCTGTTG
1514191fw	ATACTGTTTGGCAGAGGCAA
1514191rv	GGTATGGCTGATGATGTGCT
1514191p	TGACCGGTCAGCGGATCACC
2318305fw	ATAATCACCTACGCGCCTTC
2318305rv	ACCTGCCTGCCTGAATAAAC
2318305p	TGCCGCAGATCACCCTGGTC
2351285fw	ACACATTGCCGAATATGCC
2351285rv	GTTAATGCGGTGATCCATGA
2351285p	CTGCGCATTCGCATGTTCCC

1. Yamazoe M, Adachi S, Kanaya S, Ohsumi K, and Hiraga S. Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli. Mol Microbiol. 2005 Jan;55(1):289-98. DOI:10.1111/j.1365-2958.2004.04389.x |