User:Trevor W. Gruen/Notebook/Biology 210 at AU

Lab 2: Hay Infusion January 28th 2016 file:///Users/trevorgruen/Downloads/Lab%20%232%20Hay%20Infusion/Lab%20%232%20Hay%20Infusion.html

Lab 1: Transect January 28th 2016 Biotic Factors: life in the pond, shrubs, trees, microorganisms in the dirt, flowers (dead for the winter). Abiotic Factors: Rocks, concrete, benches, Roper Hall, dirt. File:Roper transect.pdf

Lab #3 Microbiology and Identifying Bacteria 2 February 2016 Trevor Gruen Hay Infusion Observations: Much of the water evaporated, and the thick moldy layer on top has largely dissipated. The smell of rot has become nearly undetectable. I would hypothesize that much of the organic matter that composed the thick top layer was food for the microorganisms in the jar, and they had consumed much of what had been there the prior week. This would explain why much of the matter and the smell were missing. Archaea likely won’t have grown on the agar plates since they prefer extreme environments, and the plate is not nearly extreme enough.

Plate Comparison: Comparing between the plates with and without antibiotic, the plates with Agar grew substantially less colonies. This indicates that the antibiotic is effective against some of the types of bacteria grown on the plates, but not all of them since some colonies grew in the higher concentration plates.

Function of Tetracycline: “Tetracyclines inhibit bacterial protein synthesis by preventing the association of aminoacyl-tRNA with the bacterial ribosome” (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99026/). Being that protein synthesis is a necessary action for all life, tetracyclines will affect any bacteria that hasn’t developed a resistance to the antibiotic.

Bio	Chem	Physics
A	A	C