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| |colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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| Objective
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| Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color.
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| [edit]BSA-AuNP
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| Start by placing all of your materials into a volumetric flask so that you know the exact volumes and exact amounts of what you have added. I will have prepared the initial protein and gold solutions for you (this one time only). In the future, you will be expected to make most of the starting solutions.
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| This procedure was taken from the following reference and has been used by our previous two Experimental Biological Chemistry groups.
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| Add 1mL of the (~2.5mM -note the exact concentration) gold (HAuCl4) solution to a 10mL volumetric flask
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| Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA
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| Add deionized water up to 10mL
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| Transfer solution to a test tube and cap with aluminum foil
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| Heat in oven at 80C for 3 hours
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| Transfer solution to a plastic falcon tube (with blue cap)
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| Stock solutions made
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| Gold solution (HAuCl4·3H2O) 0.0100g in 0.0100mL water → 2.54mM
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| BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM
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| [edit]citrate-AuNP
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| This procedure is used by Dr. Miller in her research lab - Allison Alix's notebook and is taken from this reference and can be analyzed according to this reference.
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| Take 50mL of the HAuCl4 solution from the 250mL volumetric flask. Note the concentration
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| Heat this solution to boiling while stirring
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| Add 3mL 1.5mL of 1% (w/v) sodium citrate
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| Boil solution for another 40 minutes
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| Cool to room temperature and measure the volume
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| Determine the final concentration of gold and citrate
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| Note: According to Allison, and Madeline will corroborate, we don't need to reflux the solution (which would carry out the reaction in a closed system). We should be able to bring the solution to a boil on the bench top.
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| Stock solutions made
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| Gold solution (HAuCl4·3H2O) 0.0245g in 0.2505mL water → 0.249mM
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| Sodium Citrate (Na3C6H5O7·2H2O) 0.1010g in 10.0mL → 1.01%
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