User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
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# Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0245g in 0.2505mL water → 0.249mM | # Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0245g in 0.2505mL water → 0.249mM | ||
# Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | # Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | ||
==Dilutions== | |||
The following dilutions were made in lab: | |||
{|style="width:700px" | |||
|<u>Adenosine solution concentrations (M)</u> | |||
|<u>Inosine solution concentrations (M)</u> | |||
|- | |||
|3.00x10<sup>-5</sup> | |||
|4.80x10<sup>-5</sup> | |||
|- | |||
|2.50x10<sup>-5</sup> | |||
|4.00x10<sup>-5</sup> | |||
|- | |||
|2.00x10<sup>-5</sup> | |||
|3.20x10<sup>-5</sup> | |||
|- | |||
|1.50x10<sup>-5</sup> | |||
|2.40x10<sup>-5</sup> | |||
|- | |||
|1.00x10<sup>-5</sup> | |||
|1.60x10<sup>-5</sup> | |||
|- | |||
|0.50x10<sup>-5</sup> | |||
|0.80x10<sup>-5</sup> | |||
|- | |||
|0.25x10<sup>-5</sup> | |||
|0.4x10<sup>-5</sup> | |||
|} | |||
==Data== | ==Data== |
Revision as of 02:22, 21 September 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. Using the class data, a calibration curve will be created to find the molar absorbtivity. A Grubbb's test will be done in order to determine the outliers. BSA-AuNPStart by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. This procedure was taken from the following reference.
Stock solutions made
citrate-AuNP
DilutionsThe following dilutions were made in lab:
DataAs a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. Peak Wavelength: 518 nm Absorbance at Peak: .612 In order to determine the concentration, the Absorbance at 450 is known. Abs (450): .389 Abs (518)/ Abs (450)= .612/ .389= 1.57 Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. NotesThe citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab.
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