User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
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Template for this lab is taken from Dr. Hartings. Values are altered to accurately describe the lab that was conducted on this day. The template can be found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 here] | |||
==Objective== | ==Objective== | ||
Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is | Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. | ||
==BSA-AuNP== | ==BSA-AuNP== | ||
Start by placing all of | Start by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. | ||
This procedure was taken from the following [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference] | This procedure was taken from the following [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference]. | ||
# Add | # Add 1.2mL of the (~2.54mM -note the exact concentration) gold (HAuCl<sub>4</sub>) solution to a 10mL volumetric flask | ||
# Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | # Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | ||
# Add deionized water up to | # Add deionized water up to 10.2mL | ||
# Transfer solution to a test tube and cap with aluminum foil | # Transfer solution to a test tube and cap with aluminum foil | ||
# Heat in oven at 80C for 3 hours | # Heat in oven at 80C for 3 hours | ||
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==citrate-AuNP== | ==citrate-AuNP== | ||
# Take 50mL of the HAuCl<sub>4</sub> solution from the 250mL volumetric flask. | # Take 50mL of the .249mM of HAuCl<sub>4</sub> solution from the 250mL volumetric flask. | ||
# Heat this solution to boiling while stirring | # Heat this solution to boiling while stirring | ||
# Add <strike>3mL</strike> 1.5mL of 1% (w/v) sodium citrate | # Add <strike>3mL</strike> 1.5mL of 1% (w/v) sodium citrate | ||
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# Cool to room temperature and measure the volume | # Cool to room temperature and measure the volume | ||
# Determine the final concentration of gold and citrate | # Determine the final concentration of gold and citrate | ||
<u>Stock solutions made</u> | <u>Stock solutions made</u> | ||
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# Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | # Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | ||
==Data== | |||
[[Category:Course]] | [[Category:Course]] | ||
[[Category:Miscellaneous]] | [[Category:Miscellaneous]] | ||
<br>[[Image:Absorbtionvswavelength.png]] | |||
Peak Wavelength: 518 nm | |||
Absorbance at Peak: .612 | |||
In order to determine the concentration, the Absorbance at 450 is known. | |||
Abs (450): .389 | |||
Abs (518)/ Abs (450)= .612/ .389= 1.57 | |||
Using the data found [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf here] the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. | |||
Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. | |||
==Notes== | |||
The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. | |||
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. | |||
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Revision as of 00:05, 5 October 2013
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ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. This procedure was taken from the following reference.
Stock solutions made
citrate-AuNP
Data
Absorbance at Peak: .612 In order to determine the concentration, the Absorbance at 450 is known. Abs (450): .389 Abs (518)/ Abs (450)= .612/ .389= 1.57 Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. NotesThe citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab.
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