User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Template for this lab is taken from Dr. Hartings. Values are altered to accurately describe the lab that was conducted on this day. The template can be found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 here] | Template for this lab is taken from Dr. Hartings. Values are altered to accurately describe the lab that was conducted on this day. The template can be found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 here] | ||
==Objective== | ==Objective== | ||
Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color | Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. | ||
==BSA-AuNP== | ==BSA-AuNP== | ||
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==citrate-AuNP== | ==citrate-AuNP== | ||
# Take 50mL of the .249mM of HAuCl<sub>4</sub> solution from the 250mL volumetric flask. | # Take 50mL of the .249mM of HAuCl<sub>4</sub> solution from the 250mL volumetric flask. | ||
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# Cool to room temperature and measure the volume | # Cool to room temperature and measure the volume | ||
# Determine the final concentration of gold and citrate | # Determine the final concentration of gold and citrate | ||
<u>Stock solutions made</u> | <u>Stock solutions made</u> | ||
# Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0245g in 0.2505mL water → 0.249mM | # Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0245g in 0.2505mL water → 0.249mM | ||
# Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | # Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | ||
==Data== | ==Data== | ||
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[[Category:Miscellaneous]] | [[Category:Miscellaneous]] | ||
<br>[[Image:Absorbtionvswavelength.png]] | |||
Peak Wavelength: 518 nm | Peak Wavelength: 518 nm | ||
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==Notes== | ==Notes== | ||
The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. | The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. | ||
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. | |||
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__NOTOC__ | __NOTOC__ | ||
Latest revision as of 23:16, 26 September 2017
 Biomaterials Design Lab
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 Main project pageNext entry 
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ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. This procedure was taken from the following reference.
Stock solutions made
citrate-AuNP
Data
Absorbance at Peak: .612 In order to determine the concentration, the Absorbance at 450 is known. Abs (450): .389 Abs (518)/ Abs (450)= .612/ .389= 1.57 Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. NotesThe citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab.
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