User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
(→Data) |
|||
Line 50: | Line 50: | ||
[[Category:Course]] | [[Category:Course]] | ||
[[Category:Miscellaneous]] | [[Category:Miscellaneous]] | ||
<br>[[Image:Absorbtionvswavelength.png]] | |||
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. | As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. |
Revision as of 00:03, 5 October 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. This procedure was taken from the following reference.
Stock solutions made
citrate-AuNP
DataAs a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. Peak Wavelength: 518 nm Absorbance at Peak: .612 In order to determine the concentration, the Absorbance at 450 is known. Abs (450): .389 Abs (518)/ Abs (450)= .612/ .389= 1.57 Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. NotesThe citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab.
|