User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
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This procedure was taken from the following [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference] and has been used by our previous two Experimental Biological Chemistry groups. | This procedure was taken from the following [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference] and has been used by our previous two Experimental Biological Chemistry groups. | ||
# Add | # Add 1.2mL of the (~2.54mM -note the exact concentration) gold (HAuCl<sub>4</sub>) solution to a 10mL volumetric flask | ||
# Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | # Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | ||
# Add deionized water up to | # Add deionized water up to 10.2mL | ||
# Transfer solution to a test tube and cap with aluminum foil | # Transfer solution to a test tube and cap with aluminum foil | ||
# Heat in oven at 80C for 3 hours | # Heat in oven at 80C for 3 hours |
Revision as of 13:50, 9 September 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of your materials into a volumetric flask so that you know the exact volumes and exact amounts of what you have added. I will have prepared the initial protein and gold solutions for you (this one time only). In the future, you will be expected to make most of the starting solutions. This procedure was taken from the following reference and has been used by our previous two Experimental Biological Chemistry groups.
Stock solutions made
citrate-AuNPThis procedure is used by Dr. Miller in her research lab - Allison Alix's notebook and is taken from this reference and can be analyzed according to this reference.
Stock solutions made
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