User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28: Difference between revisions
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Template for lab is taken from Dr. Hartings. Values are altered to accurately describe the lab that was conducted on this day. The template can be found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 here] | |||
==Objective== | ==Objective== | ||
Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. | Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. |
Revision as of 14:03, 9 September 2013
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ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of your materials into a volumetric flask so that you know the exact volumes and exact amounts of what you have added. I will have prepared the initial protein and gold solutions for you (this one time only). In the future, you will be expected to make most of the starting solutions. This procedure was taken from the following reference and has been used by our previous two Experimental Biological Chemistry groups.
Stock solutions made
citrate-AuNPThis procedure is used by Dr. Miller in her research lab - Allison Alix's notebook and is taken from this reference and can be analyzed according to this reference.
Stock solutions made
Notes |