User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28
|Biomaterials Design Lab|| Main project page|
Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. Using the class data, a calibration curve will be created to find the molar absorbtivity. A Grubbb's test will be done in order to determine the outliers.
Start by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings.
This procedure was taken from the following reference.
Stock solutions made
The following dilutions were made in lab:
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab.
Peak Wavelength: 518 nm
Absorbance at Peak: .612
In order to determine the concentration, the Absorbance at 450 is known.
Abs (450): .389
Abs (518)/ Abs (450)= .612/ .389= 1.57
Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8.
Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M.
The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab.