User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/08/28
|Biomaterials Design Lab|| Main project page|
Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color.
Start by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings.
This procedure was taken from the following reference.
Stock solutions made
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab.
Peak Wavelength: 518 nm
Absorbance at Peak: .612
In order to determine the concentration, the Absorbance at 450 is known.
Abs (450): .389
Abs (518)/ Abs (450)= .612/ .389= 1.57
Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8.
Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M.
The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab.