User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/03

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(Objective)
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(Dilutions)
 
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==Objective==
==Objective==
The molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine] were determined in this lab using UV-Vis and Beer's law.  
The molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine] were determined in this lab using UV-Vis and Beer's law.  
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The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. A calibration curve from the class data will be created. From this data the the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.
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The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, the molar absorptivity (ε) of both of these molecules will be known. A calibration curve from the class data will be created. From this data the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.
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Using the class data, a calibration curve will be created to find the molar absorbtivity. A Grubbb's test will be done in order to determine the outliers.
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==Dilutions==
==Dilutions==
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The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.
The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.
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The groups then exchanged unknowns to determine the concentration from the calibration curves. In a week, the data will be revisited and the error will be propagated from the calibration curve to the concentration calculation. After making the calculation, the calculation of the unknown from the group will be compared
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'''Preparation of Dilutions of Adenosine'''
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==Notes==
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[[Image:Screen_Shot_2013-10-05_at_4.20.59_AM.png]]<br>
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This area is for any observations or conclusions that you would like to note.
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'''Preparation of Dilutions of Inosine'''
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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[[Image:Screen_Shot_2013-10-05_at_3.41.48_AM.png]]<br>
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The groups then exchanged unknowns to determine the concentration from the calibration curves. In a week, the data will be revisited and the error will be propagated from the calibration curve to the concentration calculation. After making the calculation, the calculation of the unknown from the group will be compared.
[[Category:Course]]
[[Category:Course]]
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[[Category:Miscellaneous]]
[[Category:Miscellaneous]]
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==Data==
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'''Adenosine Absorbance Spectrum at 260 nm'''
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[[Image:Screen_Shot_2013-10-05_at_5.21.54_AM.png]]<br>
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'''Adenosine Calibration Curve at 260 nm
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'''
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[[Image:Screen_Shot_2013-10-05_at_4.31.20_AM.png]]<br>
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'''Class Calibration Curve for Adenosine at 259 nm'''
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[[Image:Screen_Shot_2013-10-05_at_4.43.15_AM.png]]<br>
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==Notes==
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Due to the time restrictions of the lab, the inosine spectra was taken the next lab (9/4/13).
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Grubb's test was conducted to determine the outliers in the pooled class data.
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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here

Objective

The molar absorptivities of two different molecules, adenosine and inosine were determined in this lab using UV-Vis and Beer's law. The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, the molar absorptivity (ε) of both of these molecules will be known. A calibration curve from the class data will be created. From this data the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.


.

Dilutions

Stock Solutions

Stock solutions were made to create these dilutions for each molecule. The calculations for the stock solution and dilutions were performed before lab.

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5
RANDOM RANDOM

The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.

Preparation of Dilutions of Adenosine

Image:Screen_Shot_2013-10-05_at_4.20.59_AM.png

Preparation of Dilutions of Inosine

Image:Screen_Shot_2013-10-05_at_3.41.48_AM.png
The groups then exchanged unknowns to determine the concentration from the calibration curves. In a week, the data will be revisited and the error will be propagated from the calibration curve to the concentration calculation. After making the calculation, the calculation of the unknown from the group will be compared.

Data

Adenosine Absorbance Spectrum at 260 nm

Image:Screen_Shot_2013-10-05_at_5.21.54_AM.png

Adenosine Calibration Curve at 260 nm

Image:Screen_Shot_2013-10-05_at_4.31.20_AM.png

Class Calibration Curve for Adenosine at 259 nm

Image:Screen_Shot_2013-10-05_at_4.43.15_AM.png

Notes

Due to the time restrictions of the lab, the inosine spectra was taken the next lab (9/4/13).

Grubb's test was conducted to determine the outliers in the pooled class data.


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