User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/03

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'''Class Calibration Curve for Adenosine at 259'''
'''Class Calibration Curve for Adenosine at 259 nm'''

Revision as of 05:35, 5 October 2013

Biomaterials Design Lab Main project page
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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here


The molar absorptivities of two different molecules, adenosine and inosine were determined in this lab using UV-Vis and Beer's law. The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, the molar absorptivity (ε) of both of these molecules will be known. A calibration curve from the class data will be created. From this data the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.



Stock Solutions

Stock solutions were made to create these dilutions for each molecule. The calculations for the stock solution and dilutions were performed before lab.

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5

The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.

Preparation of Dilutions of Adenosine


Preparation of Dilutions of Inosine

The groups then exchanged unknowns to determine the concentration from the calibration curves. In a week, the data will be revisited and the error will be propagated from the calibration curve to the concentration calculation. After making the calculation, the calculation of the unknown from the group will be compared


Adenosine Absorbance Spectrum at 260 nm


Adenosine Calibration Curve at 260 nm


Class Calibration Curve for Adenosine at 259 nm



Due to the time restrictions of the lab, the inosine spectra was taken the next lab (9/4/13).

Grubb's test was conducted to determine the outliers in the pooled class data.

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