User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/03

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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here

Objective

The molar absorptivities of two different molecules, adenosine and inosine were determined in this lab using UV-Vis and Beer's law. The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. A calibration curve from the class data will be created. From this data the the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.


Dilutions

Stock Solutions

Stock solutions were made to create these dilutions for each molecule. The calculations for the stock solution and dilutions were performed before lab.

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5
RANDOM RANDOM

The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.

The groups then exchanged unknowns to determine the concentration from the calibration curves. In a week, the data will be revisited and the error will be propagated from the calibration curve to the concentration calculation. After making the calculation, the calculation of the unknown from the group will be compared

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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