User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/04

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(Autocreate 2013/09/04 Entry for User:Trisha_I._Ibeh/Notebook/Trisha_Notebook)
Current revision (07:08, 5 October 2013) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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* Insert content here...
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==Objective==
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Finish up the lab from the previous day (9/3/13) by taking the UV- Vis spectrum of inosine and doing data analysis. From the data, a Grubb's test will be performed to determine the outliers. The concentration of the unknown sample will be determined ad the molar absorbtivity will also be determined from the class data.
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==Procedure==
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The same procedure that was performed with adenosine will be performed with inosine. The procedure can be found [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03|here.]]
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==Data==
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'''Inosine Abs Spectra'''
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[[Image:Screen_Shot_2013-10-05_at_6.27.27_AM.png]]<br>
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'''Inosine Calibration Curve'''
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[[Image: Screen_Shot_2013-10-05_at_6.13.30_AM.png]]<br>
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'''Class Data (pooled)'''
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[[Image: Screen_Shot_2013-10-05_at_6.57.56_AM.png]] <br>
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[[Image: Screen_Shot_2013-10-05_at_6.31.40_AM.png]]<br>
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'''Abs mean and std dev (adenosine and inosine)'''
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'''Absorbance Spectra of Unknown'''
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[[Image: Screen_Shot_2013-10-05_at_7.07.13_AM.png]]<br>
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==Notes==
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I made a stock solution of BSA today. Here is the info:
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BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM
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[[Category:Course]]
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[[Category:Miscellaneous]]
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[[Category:Course]]
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[[Category:Miscellaneous]]
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Current revision

Biomaterials Design Lab Main project page
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Objective

Finish up the lab from the previous day (9/3/13) by taking the UV- Vis spectrum of inosine and doing data analysis. From the data, a Grubb's test will be performed to determine the outliers. The concentration of the unknown sample will be determined ad the molar absorbtivity will also be determined from the class data.

Procedure

The same procedure that was performed with adenosine will be performed with inosine. The procedure can be found here.

Data

Inosine Abs Spectra

Image:Screen_Shot_2013-10-05_at_6.27.27_AM.png

Inosine Calibration Curve

Image: Screen_Shot_2013-10-05_at_6.13.30_AM.png

Class Data (pooled)

Image: Screen_Shot_2013-10-05_at_6.57.56_AM.png

Image: Screen_Shot_2013-10-05_at_6.31.40_AM.png

Abs mean and std dev (adenosine and inosine)

Absorbance Spectra of Unknown

Image: Screen_Shot_2013-10-05_at_7.07.13_AM.png

Notes

I made a stock solution of BSA today. Here is the info:

BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM




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