User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/04
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Become familiar with the fluorescence spectrometer and with protein fluorescence.
Fluorescence spectroscopy is another way to analyze molecular samples.
Each group will pick a different protein (bovine serum albumin, horseradish peroxidase, pepsin, adenosine deaminase, hemoglobin). You'll need to make 4 samples ranging from 10uM to 0.5uM. Remember, we don't have a lot of most of these proteins, so figure out how to make a stock solution using as little solid protein as possible. The fluorescence cuvette needs only 200uL, so you don't need to make more than that for each concentration that you're measuring.
I made a stock solution of BSA today. Here is the info:
BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM