User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/04
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Finish up the lab from the previous day (9/3/13) by taking the UV- Vis spectrum of inosine and doing data analysis. From the data, a Grubb's test will be performed to determine the outliers. The concentration of the unknown sample will be determined ad the molar absorbtivity will also be determined from the class data.
The same procedure that was performed with adenosine will be performed with inosine. The procedure can be found here.
Each group will pick a different protein (bovine serum albumin, horseradish peroxidase, pepsin, adenosine deaminase, hemoglobin). You'll need to make 4 samples ranging from 10uM to 0.5uM. Remember, we don't have a lot of most of these proteins, so figure out how to make a stock solution using as little solid protein as possible. The fluorescence cuvette needs only 200uL, so you don't need to make more than that for each concentration that you're measuring.
I made a stock solution of BSA today. Here is the info:
BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM