User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/10
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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here
Make a calibration curve for different proteins that will be used during the semester with the Bradford Assay.The protein of choice was bovine serum albumin (BSA)and using the procedure provided above, 6 different standards were created and analyzed using UV- Vis.
The basic protocol can be found here. (*Note: use section 2.3, page 5)
- Note: This is where coordination is a good thing. Take 1 spectrum at a time. Let other people go.
The Atomic Absorption standards will be made for the following day.
Using the gold AA/ICPMS standard solution make 5 new solutions (Note: Use water - NOT BUFFER - to make these solutions)