User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/22: Difference between revisions

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## Place tubes in oven for 4 hours at 80 degrees Celsius
## Place tubes in oven for 4 hours at 80 degrees Celsius
##Take the UV Vis of BSA, Au, HRP, and Pepsin
##Take the UV Vis of BSA, Au, HRP, and Pepsin
Calculations used to make a solutions.
[[Image:Screen_Shot_2013-11-03_at_4.18.22_AM.png]]


==Data==
==Data==

Revision as of 02:24, 3 November 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here

Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Procedure

  1. Make solutions in test tube that will contain a total of 5mL add
    1. Au solution such that the final concentration is 0.25mM
    2. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    3. Water up to 5mL
    4. Place tubes in oven for 4 hours at 80 degrees Celsius
    5. Take the UV Vis of BSA, Au, HRP, and Pepsin

Calculations used to make a solutions.


Data

UV- Vis of stock solutions used to make samples.





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