User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/23: Difference between revisions
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==Procedure== | ==Procedure== | ||
# Protein-AuNP analysis | |||
## Take a picture and note physical description of each ratio | |||
## Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
## Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.) | |||
## Take a UV-Vis spectrum of each sample (Be considerate of other users here) | |||
## After taking the spectrum, save the sample in a fresh eppy tube. | |||
# Synthesis of HRP-AuNPs and Hemoglobin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## For Myoglobin make the following solutions to reflect an Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## For HRP make the following solutions to reflect an Au:Protein ratios | |||
### 230 | |||
### 250 | |||
### 270 | |||
### 290 | |||
### 310 | |||
### 330 | |||
### 350 | |||
### 370 | |||
### 390 | |||
### 410 | |||
## Water up to 5mL | |||
==Data== | ==Data== |
Revision as of 03:36, 3 November 2013
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Objective
Procedure
Data |