User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/23: Difference between revisions
From OpenWetWare
(→Data) |
(→Notes) |
||
(11 intermediate revisions by the same user not shown) | |||
Line 8: | Line 8: | ||
==Objective== | ==Objective== | ||
*Measure pepsin activity of pepsin in solution and pepsin-AuNPs in solution | |||
*Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday | |||
*Synthesize HRP- AuNP at higher ratios. | |||
*Synthesize Myoglobin- AuNPs with the ratio used in yesterday's lab | |||
==Procedure== | |||
# Protein-AuNP analysis | |||
## Take a picture and note physical description of each ratio | |||
## Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
## Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.) | |||
## Take a UV-Vis spectrum of each sample (Be considerate of other users here) | |||
## After taking the spectrum, save the sample in a fresh eppy tube. | |||
# Synthesis of HRP-AuNPs and Hemoglobin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## For Myoglobin make the following solutions to reflect an Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## For HRP make the following solutions to reflect an Au:Protein ratios | |||
### 230 | |||
### 250 | |||
### 270 | |||
### 290 | |||
### 310 | |||
### 330 | |||
### 350 | |||
### 370 | |||
### 390 | |||
### 410 | |||
## Water up to 5mL | |||
##Place solutions in oven for 4 hours at 80 degrees Celsius | |||
Calculations used to make solutions | |||
[[Image:Screen_Shot_2013-11-03_at_5.38.05_AM.png]] | |||
==Data== | ==Data== | ||
Line 17: | Line 62: | ||
[[Image: Screen_Shot_2013-11-03_at_5.24.59_AM.png]] | [[Image: Screen_Shot_2013-11-03_at_5.24.59_AM.png]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | [[Image:Screen_Shot_2013-11-03_at_5.26.21_AM.png]] | ||
==Notes== | |||
Observations | |||
[[HRP]] | |||
*Purple tint of varying degrees:Lower ratios were magenta and higher ratios were blueish purple) | |||
*No fibers formed | |||
[[Pepsin]] | |||
*Fibers not formed | |||
<u>Stock Solutions</u> | |||
# Gold | |||
## 49.3 mg HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water ---> 2.50mM | |||
# HRP | |||
## 6.6 mg HRP in 100 mL water ---> 1.5μM | |||
# Myoglobin | |||
## 28.7mg myoglobin in 100mL water ---> 16.9μM<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | |||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 03:55, 3 November 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Procedure
DataNotesObservations
|