# User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/23

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Calculations used to make solutions | Calculations used to make solutions |

## Revision as of 05:41, 3 November 2013

Biomaterials Design Lab | Main project page Previous entry Next entry |

## Objective- Measure pepsin activity of pepsin in solution and pepsin-AuNPs in solution
- Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday
- Synthesize HRP- AuNP at higher ratios.
- Synthesize Myoglobin- AuNPs with the ratio used in yesterday's lab
## Procedure- Protein-AuNP analysis
- Take a picture and note physical description of each ratio
- Place 1mL of each ratio in an 1.5mL eppendorf tube.
- Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.)
- Take a UV-Vis spectrum of each sample (Be considerate of other users here)
- After taking the spectrum, save the sample in a fresh eppy tube.
- Synthesis of HRP-AuNPs and Hemoglobin-AuNPs
- Into test tube that will contain a total of 5mL add
- Au solution such that the final concentration is 0.25mM
- For Myoglobin make the following solutions to reflect an Au:Protein ratios
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- For HRP make the following solutions to reflect an Au:Protein ratios
- 230
- 250
- 270
- 290
- 310
- 330
- 350
- 370
- 390
- 410
- Water up to 5mL
- Place solutions in oven for 4 hours at 80 degrees Celsius
Calculations used to make solutions ## Data |