User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/29: Difference between revisions
From OpenWetWare
No edit summary |
|||
| Line 15: | Line 15: | ||
Perform Atomic Absorption on the proteins from the previous two labs ( [HRP-AuNPs and pepsin-AuNPs] and [Myoglobin-AuNPs and HRP-AuNPs]). | Perform Atomic Absorption on the proteins from the previous two labs ( [HRP-AuNPs and pepsin-AuNPs] and [Myoglobin-AuNPs and HRP-AuNPs]). | ||
==Procedure== | |||
# Before any measurement, take note of physical appearance of your samples (taking pics with cell phones helps here) | |||
# Atomic Absorption | |||
## centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm. | |||
## make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL | |||
## Take measurements of your standards and samples | |||
## Make a graph showing concentration of Au vs Au:protein ratio | |||
__NOTOC__ | __NOTOC__ | ||
Revision as of 01:02, 3 November 2013
 Biomaterials Design Lab
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here
Objective
Perform Atomic Absorption on the proteins from the previous two labs ( [HRP-AuNPs and pepsin-AuNPs] and [Myoglobin-AuNPs and HRP-AuNPs]).
Procedure
- Before any measurement, take note of physical appearance of your samples (taking pics with cell phones helps here)
- Atomic Absorption
- centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
- make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
- Take measurements of your standards and samples
- Make a graph showing concentration of Au vs Au:protein ratio
