User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/01/29: Difference between revisions
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==Objective== | |||
==Procedure== | |||
==Magnetic Nanoparticles Day 6== | ==Magnetic Nanoparticles Day 6== | ||
*Mag BSA-AuNPs were created and bubbled through with nitrogen | *Mag BSA-AuNPs were created and bubbled through with nitrogen | ||
*UV Vis was performed on the samples made in the previous lab | *UV Vis was performed on the samples made in the previous lab | ||
Create magnetic BSA-iron NPS | |||
# Synthesize BSA- iron NPs into test tube that will contain a total of 5mL | |||
# BSA- iron solution that has final ratio of 130:1 | |||
# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3 | |||
# Water up to 5mL | |||
# De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius | |||
Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of: | |||
#125:1 | |||
#130:1 | |||
#135:1 | |||
==Notes== | ==Notes== |
Revision as of 00:14, 1 May 2014
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ObjectiveProcedureMagnetic Nanoparticles Day 6
Create magnetic BSA-iron NPS
NotesHemoglobin could not dissolve in water therefore PIs continued with BSA
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