User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18

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(Sample Prep for Stock Solutions)
Current revision (06:34, 7 May 2014) (view source)
(Preparation of Magnetic Nanoparticles)
 
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#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles  
#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles  
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
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==Atomic Absorption Prep==
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Table 1: Prep of Standard Iron Samples
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[[Image: Screen_Shot_2014-05-07_at_6.30.22_AM.png ]]
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Table 2: Calibration Curve Data
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[[Image: Screen_Shot_2014-05-07_at_6.30.12_AM.png]]
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Table 3: Atomic Absorption Data
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[[Image: Screen_Shot_2014-05-07_at_6.30.28_AM.png ]]
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Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Image: Screen_Shot_2014-05-07_at_4.12.08_AM.png

Table 2: FeSO4 Stock Prep

Image: Screen_Shot_2014-05-07_at_4.16.17_AM.png

Table 3: Hemoglobin Prep

Image:Screen Shot 2014-05-07 at 4.12.28 AM.png

Table 4: KNO3 Prep

Image: Screen_Shot_2014-05-07_at_4.12.36_AM.png

Table 5: KOH Prep

Image: Screen_Shot_2014-05-07_at_4.12.43_AM.png

Table 6: Gold Prep

Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png

Table 7: Mag: BSA Prep

Image: Screen_Shot_2014-05-07_at_6.24.36_AM.png

Table 8: Mag: Hemoglobin Prep

Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png

Table 9: Gold: BSA Prep

Image: Screen_Shot_2014-05-07_at_6.27.17_AM.png

Table 10: Gold: Hemoglobin Prep

Image: Screen_Shot_2014-05-07_at_6.22.10_AM.png

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius

Atomic Absorption Prep

Table 1: Prep of Standard Iron Samples

Image: Screen_Shot_2014-05-07_at_6.30.22_AM.png

Table 2: Calibration Curve Data

Image: Screen_Shot_2014-05-07_at_6.30.12_AM.png

Table 3: Atomic Absorption Data

Image: Screen_Shot_2014-05-07_at_6.30.28_AM.png


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