User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18

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(Sample Prep for Stock Solutions)
(Sample Prep for Stock Solutions)
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Table 7: Mag: BSA Prep
Table 7: Mag: BSA Prep
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Table 8: Mag: Hemoglobin Prep
Table 8: Mag: Hemoglobin Prep
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Table 9: Gold: BSA Prep
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[[Image: Screen_Shot_2014-05-07_at_6.27.17_AM.png ]]
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Table 10: Gold: Hemoglobin Prep
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==Preparation of Magnetic Nanoparticles==
==Preparation of Magnetic Nanoparticles==

Revision as of 06:28, 7 May 2014

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Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Image: Screen_Shot_2014-05-07_at_4.12.08_AM.png

Table 2: FeSO4 Stock Prep

Image: Screen_Shot_2014-05-07_at_4.16.17_AM.png

Table 3: Hemoglobin Prep

Image:Screen Shot 2014-05-07 at 4.12.28 AM.png

Table 4: KNO3 Prep

Image: Screen_Shot_2014-05-07_at_4.12.36_AM.png

Table 5: KOH Prep

Image: Screen_Shot_2014-05-07_at_4.12.43_AM.png

Table 6: Gold Prep

Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png

Table 7: Mag: BSA Prep

Image: Screen_Shot_2014-05-07_at_6.24.36_AM.png

Table 8: Mag: Hemoglobin Prep

Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png

Table 9: Gold: BSA Prep

Image: Screen_Shot_2014-05-07_at_6.27.17_AM.png

Table 10: Gold: Hemoglobin Prep

Image: Screen_Shot_2014-05-07_at_6.22.10_AM.png

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius


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