User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18

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Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Table 2: FeSO4 Stock Prep

Table 3: Hemoglobin Prep

Table 4: KNO3 Prep

Table 5: KOH Prep

Table 6: Gold Prep

Table 7: Mag: BSA Prep

Table 8: Mag: Hemoglobin Prep

Table 9: Gold: BSA Prep

Table 10: Gold: Hemoglobin Prep

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius

Atomic Absorption Prep

Table 1: Prep of Standard Iron Samples

Table 2: Calibration Curve Data

Table 3: Atomic Absorption Data


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