User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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[[Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png]]
[[Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png]]
Table 7: Mag: BSA Prep
[[Image: Screen_Shot_2014-05-07_at_6.24.36_AM.png]]
Table 8: Mag: Hemoglobin Prep
[[Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png ]]
Table 9: Gold: BSA Prep
[[Image: Screen_Shot_2014-05-07_at_6.27.17_AM.png ]]
Table 10: Gold: Hemoglobin Prep
[[Image: Screen_Shot_2014-05-07_at_6.22.10_AM.png]]
==Preparation of Magnetic Nanoparticles==
#Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
#Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
==Atomic Absorption Prep==
Table 1: Prep of Standard Iron Samples
[[Image: Screen_Shot_2014-05-07_at_6.30.22_AM.png ]]
Table 2: Calibration Curve Data
[[Image: Screen_Shot_2014-05-07_at_6.30.12_AM.png]]
Table 3: Atomic Absorption Data
[[Image: Screen_Shot_2014-05-07_at_6.30.28_AM.png ]]
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Latest revision as of 23:57, 26 September 2017

Project name Main project page
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Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Table 2: FeSO4 Stock Prep

Table 3: Hemoglobin Prep

Table 4: KNO3 Prep

Table 5: KOH Prep

Table 6: Gold Prep

Table 7: Mag: BSA Prep

Table 8: Mag: Hemoglobin Prep

Table 9: Gold: BSA Prep

Table 10: Gold: Hemoglobin Prep

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius

Atomic Absorption Prep

Table 1: Prep of Standard Iron Samples

Table 2: Calibration Curve Data

Table 3: Atomic Absorption Data


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